小鼠补体第五成分的性别二态性。
Sexual dimorphism of the fifth component of mouse complement.
作者信息
Baba A, Fujita T, Tamura N
出版信息
J Exp Med. 1984 Aug 1;160(2):411-9. doi: 10.1084/jem.160.2.411.
Abstract
Sexual dimorphism of mouse complement component 5 (C5) was detected by isoelectric focusing of desialated ethylenediamine tetraacetic acid (EDTA)-plasma on agarose gel, followed by immunofixation with anti-mouse C5. Male plasma displayed two C5 bands, basic and acidic, while female plasma had only a basic C5 band. In all mouse strains tested except C5-deficient strains, the identical patterns of the dimorphism were obtained. The basic and acidic C5 were antigenically and hemolytically indistinguishable from each other. In the neonatal mice of both sexes, the basic C5 was observed at the same levels, but the acidic C5 was only slightly detected. Injection of testosterone into mice resulted in an appearance of the acidic C5 in females and its increase in males. By contrast, injection of estradiol decreased the acidic C5. These results indicate that the expression of the acidic C5 is under the control of testosterone.
摘要
通过在琼脂糖凝胶上对去唾液酸化乙二胺四乙酸（EDTA）血浆进行等电聚焦，随后用抗小鼠C5进行免疫固定，检测到小鼠补体成分5（C5）的性别二态性。雄性血浆显示出两条C5带，即碱性和酸性带，而雌性血浆只有一条碱性C5带。在除C5缺陷型品系外的所有测试小鼠品系中，均获得了相同的二态性模式。碱性C5和酸性C5在抗原性和溶血活性上彼此无法区分。在两性新生小鼠中，碱性C5水平相同，但酸性C5仅略有检测到。给小鼠注射睾酮会导致雌性小鼠出现酸性C5，雄性小鼠酸性C5增加。相比之下，注射雌二醇会降低酸性C5。这些结果表明，酸性C5的表达受睾酮控制。
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本文引用的文献
1
DISTRIBUTION, INHERITANCE, AND PROPERTIES OF AN ANTIGEN, MUB1, AND ITS RELATION TO HEMOLYTIC COMPLEMENT.抗原MUB1的分布、遗传特性及其与溶血补体的关系
J Exp Med. 1964 Nov 1;120(5):897-924. doi: 10.1084/jem.120.5.897.
2
Mouse complement: the effect of sex hormones and castration on two of the late-acting components.小鼠补体：性激素和去势对两种晚期作用成分的影响。
J Exp Med. 1967 Apr 1;125(4):657-72. doi: 10.1084/jem.125.4.657.
3
Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum.豚鼠血清中溶血补体九种成分的分离、纯化及测定方法
Immunochemistry. 1966 Mar;3(2):111-35. doi: 10.1016/0019-2791(66)90292-8.
4
Hormonal control of MuBl concentration.MuB1浓度的激素调控。
Proc Soc Exp Biol Med. 1966 Jul;122(3):779-82. doi: 10.3181/00379727-122-31250.
5
Gene dosage effect of serum concentration of a complement component, MuBl.补体成分MuBl血清浓度的基因剂量效应
Proc Soc Exp Biol Med. 1966 Jul;122(3):775-8. doi: 10.3181/00379727-122-31249.
6
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
7
8
A sex-limited serum protein variant in the mouse: hormonal control of phenotypic expression.小鼠中的一种性别限制血清蛋白变体：表型表达的激素控制。
Biochem Genet. 1971 Apr;5(2):201-9. doi: 10.1007/BF00485645.
9
10
A method of trace iodination of proteins for immunologic studies.一种用于免疫学研究的蛋白质微量碘化方法。
Int Arch Allergy Appl Immunol. 1966;29(2):185-9. doi: 10.1159/000229699.
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