Baba A, Fujita T, Tamura N
J Exp Med. 1984 Aug 1;160(2):411-9. doi: 10.1084/jem.160.2.411.
Sexual dimorphism of mouse complement component 5 (C5) was detected by isoelectric focusing of desialated ethylenediamine tetraacetic acid (EDTA)-plasma on agarose gel, followed by immunofixation with anti-mouse C5. Male plasma displayed two C5 bands, basic and acidic, while female plasma had only a basic C5 band. In all mouse strains tested except C5-deficient strains, the identical patterns of the dimorphism were obtained. The basic and acidic C5 were antigenically and hemolytically indistinguishable from each other. In the neonatal mice of both sexes, the basic C5 was observed at the same levels, but the acidic C5 was only slightly detected. Injection of testosterone into mice resulted in an appearance of the acidic C5 in females and its increase in males. By contrast, injection of estradiol decreased the acidic C5. These results indicate that the expression of the acidic C5 is under the control of testosterone.
通过在琼脂糖凝胶上对去唾液酸化乙二胺四乙酸（EDTA）血浆进行等电聚焦，随后用抗小鼠C5进行免疫固定，检测到小鼠补体成分5（C5）的性别二态性。雄性血浆显示出两条C5带，即碱性和酸性带，而雌性血浆只有一条碱性C5带。在除C5缺陷型品系外的所有测试小鼠品系中，均获得了相同的二态性模式。碱性C5和酸性C5在抗原性和溶血活性上彼此无法区分。在两性新生小鼠中，碱性C5水平相同，但酸性C5仅略有检测到。给小鼠注射睾酮会导致雌性小鼠出现酸性C5，雄性小鼠酸性C5增加。相比之下，注射雌二醇会降低酸性C5。这些结果表明，酸性C5的表达受睾酮控制。
