Onoé K, Yasumizu R, Oh-Ishi T, Kakinuma M, Good R A, Morikawa K
J Exp Med. 1981 Apr 1;153(4):1009-14. doi: 10.1084/jem.153.4.1009.
Employing a new method for allogeneic bone marrow transplantation, irradiation chimeras constructed from various combinations of marrow cells from B10 H-2 recombinant mice and AKR recipients were prepared. Though these chimeras had well-developed populations of T and B cells, they showed strikingly different patterns of responses in the primary antibody formation to sheep erythrocytes (SRBC), a T dependent antigen. These are (a) AKR mice treated with C57BL/10 cells, [B10 leads to AKR] fully H-2 incompatible, and AKR mice treated with B10.A (5R) cells, [5R leads to AKR] I-J,E compatible chimeras that were almost completely unresponsive to SRBC; (b) AKR mice treated with B10.BR cells [BR leads to AKR] fully H-2 compatible, and AKR mice treated with B10 AKM cells, [AKM leads to AKR] chimeras where donor and recipient differed only at H-2D, showed the same number of plaque-forming cells (PFC) as B10 control mice; (c) AKR mice treated with B10.A cells, [B10 leads to AKR] chimeras, where donor and recipient were matched at H-2K-I-E region, showed about one-half the number of PFC as the control mice. From these results we conclude that in allogeneic bone marrow chimeras primary antibody response to T-dependent antigen, such as SRBC, is generated when at least the K end of the H-2 complex is compatible between donor and recipient.
采用一种新的同种异体骨髓移植方法，制备了由B10 H-2重组小鼠的骨髓细胞与AKR受体的各种组合构建的辐射嵌合体。尽管这些嵌合体具有发育良好的T细胞和B细胞群体，但它们在对绵羊红细胞（SRBC，一种T细胞依赖性抗原）的初次抗体形成反应中表现出截然不同的模式。这些模式如下：（a）用C57BL/10细胞处理的AKR小鼠，[B10→AKR]完全H-2不相容，以及用B10.A（5R）细胞处理的AKR小鼠，[5R→AKR] I-J、E相容的嵌合体，它们对SRBC几乎完全无反应；（b）用B10.BR细胞处理的AKR小鼠[BR→AKR]完全H-2相容，以及用B10 AKM细胞处理的AKR小鼠，[AKM→AKR]供体和受体仅在H-2D处不同的嵌合体，其形成空斑细胞（PFC）的数量与B10对照小鼠相同；（c）用B10.A细胞处理的AKR小鼠，[B10→AKR]嵌合体，其供体和受体在H-2K-I-E区域匹配，其PFC数量约为对照小鼠的一半。从这些结果我们得出结论，在同种异体骨髓嵌合体中，对T细胞依赖性抗原如SRBC的初次抗体反应，当供体和受体之间至少H-2复合体的K端相容时产生。
