Ali Ahmed Qasem, Farah Mohammad Abul, Abou-Tarboush Faisal M, Al-Anazi Khalid M, Ali M Ajmal, Lee Joongku, Hailan Waleed A Q, Mahmoud Ahmed Hossam
Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci. 2019 Mar;26(3):547-553. doi: 10.1016/j.sjbs.2018.12.014. Epub 2018 Dec 30.
The extracts prepared from various areal parts of the (Forssk.) Roem. & Schult. (Family: Apocynaceae) including leaves, fruit and seeds ethanolic extracts and seed aqueous extract were evaluated against MCF-7 cells in order to investigate its potential of cytogenotoxicity and induction of apoptosis. The ethanolic seeds extract had comparatively higher cytotoxicity (IC ∼ 337 µg/ml). Further, apoptosis and DNA damaging potential of seeds ethanolic extract was analyzed by applying multiple sub-lethal concentrations and durations. Flow cytometry results revealed that maximum percentage of early apoptosis (37%) and late apoptosis (35%) were observed after 12 h exposure in concentrations 200 µg/ml and 300 µg/ml, respectively. Similarly, the higher effect of extract in terms of DNA damage by alkaline comet assay was registered after 12 h treatment at concentrations 200 and 300 µg/mL. The calculated total damage score (TDS) for these concentrations were 614 and 617, respectively. The above findings indicate that ethanolic seeds extracts has cytogenotoxic properties that could be further explored for the potential source of chemotherapeutic lead against cancer.
对萝芙木（Forssk.）Roem. & Schult.（夹竹桃科）不同部位制备的提取物，包括叶、果实和种子的乙醇提取物以及种子水提取物，针对MCF - 7细胞进行了评估，以研究其细胞遗传毒性和诱导细胞凋亡的潜力。种子乙醇提取物具有相对较高的细胞毒性（IC₅₀ ∼ 337 μg/ml）。此外，通过应用多个亚致死浓度和作用时间，分析了种子乙醇提取物的凋亡和DNA损伤潜力。流式细胞术结果显示，在分别暴露于200 μg/ml和300 μg/ml浓度12小时后，观察到早期凋亡的最大百分比为37%，晚期凋亡的最大百分比为35%。同样，在200和300 μg/mL浓度下处理12小时后，碱性彗星试验显示提取物对DNA损伤的影响更大。这些浓度下计算出的总损伤评分（TDS）分别为614和617。上述发现表明，萝芙木种子乙醇提取物具有细胞遗传毒性特性，可进一步探索其作为抗癌化疗先导潜在来源的可能性。
