Gupta Neha, Bhagyawant Sameer Suresh
School of Studies in Biotechnology, Jiwaji University, Gwalior, 474011 Madhya Pradesh India.
3 Biotech. 2021 Feb;11(2):63. doi: 10.1007/s13205-020-02614-6. Epub 2021 Jan 12.
Chickpea seed proteins are alleged source of nutraceuticals. These seed proteins were subjected to different proteases to produce peptides. The efficacy of these peptides was confirmed using six diverse human cancer cell lines (PA-1, Ishikawa cells, A549, MCF-7, HepG2, MDA-MB-231). Alcalase generated peptides exhibited the highest antagonistic inhibition of Ishikawa cells. Flow cytometric analysis revealed that chickpea peptide induced S and G2 phase arrest of cell cycle in a dose dependent manner. DNA fragmentation and apoptosis occurred by down regulation of Bcl-2 expression, upregulation of expression and promotion of caspase-3 initiation. Chickpea peptides ascertain potential antiproliferative molecule that can be deployed in cancer treatment regimes.
鹰嘴豆种子蛋白被认为是营养保健品的来源。这些种子蛋白经过不同的蛋白酶处理以产生肽。使用六种不同的人类癌细胞系（PA - 1、石川细胞、A549、MCF - 7、HepG2、MDA - MB - 231）证实了这些肽的功效。碱性蛋白酶产生的肽对石川细胞表现出最高的拮抗抑制作用。流式细胞术分析表明，鹰嘴豆肽以剂量依赖性方式诱导细胞周期的S期和G2期阻滞。DNA片段化和细胞凋亡通过下调Bcl - 2表达、上调[此处原文缺失相关基因名称]表达以及促进caspase - 3启动而发生。鹰嘴豆肽确定了可用于癌症治疗方案的潜在抗增殖分子。
