Ohkubo M, Sawada K, Sakiyama S
Jpn J Cancer Res. 1986 Jun;77(6):546-53.
When proteins in the tissue extracts of lung cancers were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE), a 37,000-dalton protein (37K) was detected as a prominent form in all cases thus far examined. This protein was barely detectable or its amount was very small in the normal lung tissue extracts. Two-dimensional PAGE of lung tumor extracts showed 37K as a major spot with an isoelectric point of 7.6. An antibody against 37K was raised in a rabbit by injecting purified 37K. Immunoblot analysis of normal lung and lung tumor tissues revealed that the affinity-purified anti-37K antibody specifically recognized 37K and that the intensities of immunostaining corresponded to those of Coomassie brilliant blue staining.
当通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(PAGE)分析肺癌组织提取物中的蛋白质时,在迄今为止检测的所有病例中,一种37,000道尔顿的蛋白质(37K)被检测为主要形式。这种蛋白质在正常肺组织提取物中几乎检测不到或含量非常少。肺肿瘤提取物的二维PAGE显示37K是一个主要斑点,等电点为7.6。通过注射纯化的37K在兔体内产生了针对37K的抗体。对正常肺组织和肺肿瘤组织的免疫印迹分析表明,亲和纯化的抗37K抗体特异性识别37K,并且免疫染色强度与考马斯亮蓝染色强度一致。