Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China.
Department of Nuclear Medicine, Peking Union Medical Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China.
Sci China Life Sci. 2019 Jun;62(6):791-806. doi: 10.1007/s11427-018-9495-2. Epub 2019 Mar 13.
Pancreatic cancer is one of the most lethal human malignancies, partly because of its propensity for metastasis. However, highly metastatic human pancreatic cancer cell lines suitable for studies of metastasis are currently lacking. Here we established two highly metastatic human pancreatic cancer cell lines, MIA PaCa-2 In8 and Panc-1 In8, by Matrigel induction assay. The cell lines were further characterized both in vitro and in vivo. MIA PaCa-2 In8 and Panc-1 In8 cells demonstrated increased migration and invasion compared with their respective parental cells. Following injection into nude mice, MIA PaCa-2 In8 and Panc-1 In8 cells resulted in more pulmonary metastases compared with the parental cells. Furthermore, analyses of mRNA, long non-coding RNA, micro RNA, and methylation profiling revealed that these factors were aberrantly regulated in the highly metastatic cells, indicating that they probably affected metastasis. We thus established and characterized two highly metastatic human pancreatic cell lines that could be used as valuable tools for future investigations into the pathogenesis, metastasis, and potential treatment of human pancreatic cancer.
胰腺癌是最致命的人类恶性肿瘤之一，部分原因是其易于转移。然而，目前缺乏适合转移研究的高度转移性人胰腺癌细胞系。在这里，我们通过 Matrigel 诱导实验建立了两个高度转移性的人胰腺癌细胞系，MIA PaCa-2 In8 和 Panc-1 In8。我们进一步对这些细胞系进行了体内外的特征描述。与各自的亲本细胞相比，MIA PaCa-2 In8 和 Panc-1 In8 细胞表现出更强的迁移和侵袭能力。将这些细胞系注射到裸鼠体内后，与亲本细胞相比，MIA PaCa-2 In8 和 Panc-1 In8 细胞导致更多的肺转移。此外，对 mRNA、长非编码 RNA、microRNA 和甲基化谱的分析表明，这些因子在高度转移性细胞中异常调节，表明它们可能影响转移。因此，我们建立并鉴定了两个高度转移性的人胰腺癌细胞系，它们可以作为未来研究人类胰腺癌发病机制、转移和潜在治疗的有价值的工具。
