电压钳制的青蛙神经肌肉接头处剂量-反应关系的分析。
An analysis of the dose-response relationship at voltage-clamped frog neuromuscular junctions.
作者信息
Dionne V E, Steinbach J H, Stevens C F
出版信息
J Physiol. 1978 Aug;281:421-44. doi: 10.1113/jphysiol.1978.sp012431.
Abstract
- Frog neuromuscular junctions were viewed with Nomarski optics and voltage clamped. Agonist was applied ionophoretically and agonist concentrations were measured using a micro-electrode sensitive to quaternary amines. 2. The dose-response relationship was studied using the agonists carbamylcholine, suberyldicholine and hydroxyphenyl-propyltrimethylammonium. 3. With all of these agonists, it appeared that the ACh receptor could be active when either one or two agonist binding sites were occupied. The receptor was much more likely to be active when both sites were occupied. Agonist dissociation constants and receptor activation probabilities were estimated by non-linear regression techniques for several possible receptor activation schemes.
摘要
- 用诺马斯基光学显微镜观察青蛙神经肌肉接头，并进行电压钳制。通过离子电泳施加激动剂，并使用对季胺敏感的微电极测量激动剂浓度。2. 使用激动剂氨甲酰胆碱、辛二酰胆碱和羟苯基丙基三甲基铵研究剂量反应关系。3. 对于所有这些激动剂，似乎当一个或两个激动剂结合位点被占据时，乙酰胆碱受体就可能被激活。当两个位点都被占据时，受体更有可能被激活。通过非线性回归技术对几种可能的受体激活方案估计激动剂解离常数和受体激活概率。
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