青蛙神经肌肉接头处的双组分脱敏作用
Two-component desensitization at the neuromuscular junction of the frog.
作者信息
Chesnut T J
出版信息
J Physiol. 1983 Mar;336:229-41. doi: 10.1113/jphysiol.1983.sp014578.
Abstract
Desensitization to ionophoretically applied ACh has been studied at voltage-clamped neuromuscular junctions of Rana pipiens. The time courses of both the onset of and recovery from desensitization displayed two components. The application of metabolic inhibitors to the preparation decreased the degree of recovery and also decreased both time constants of onset. The effect on onset was more pronounced on the slow component while no effect selective for either of the two components of recovery was seen. Intracellular injection of EGTA into the muscle cell increased both time constants of desensitization onset. The effect was much more pronounced on the slow component. Increasing the dose of ACh selectively decreased the fast time constant of desensitization onset. The effect was more selective in low-calcium than normal Ringer solution. These observations suggest that there are at least two independent mechanisms in the process of desensitization at the neuromuscular junction. These mechanisms differ in time course, degree of dependency on [Ca]i, and sensitivity to acetylcholine dose.
摘要
在牛蛙的电压钳制神经肌肉接头处，对离子电泳施加的乙酰胆碱（ACh）脱敏现象进行了研究。脱敏起始和恢复的时间进程均显示出两个成分。向标本施加代谢抑制剂会降低恢复程度，同时也会降低起始的两个时间常数。对起始的影响在慢成分上更为明显，而在恢复的两个成分中未观察到对其中任何一个成分有选择性的影响。向肌肉细胞内注射乙二醇双乙醚二胺四乙酸（EGTA）会增加脱敏起始的两个时间常数。该影响在慢成分上更为显著。增加乙酰胆碱的剂量会选择性地降低脱敏起始的快速时间常数。在低钙林格氏液中，该影响比在正常林格氏液中更具选择性。这些观察结果表明，在神经肌肉接头处的脱敏过程中至少存在两种独立的机制。这些机制在时间进程、对细胞内钙离子浓度（[Ca]i）的依赖程度以及对乙酰胆碱剂量的敏感性方面存在差异。
相似文献
1
Two-component desensitization at the neuromuscular junction of the frog.青蛙神经肌肉接头处的双组分脱敏作用
J Physiol. 1983 Mar;336:229-41. doi: 10.1113/jphysiol.1983.sp014578.
2
Desensitization and recovery at the frog neuromuscular junction.青蛙神经肌肉接头处的脱敏与恢复
J Gen Physiol. 1977 Apr;69(4):431-47. doi: 10.1085/jgp.69.4.431.
3
Desensitization of the acetylcholine receptor of frog end-plates measured in a Vaseline-gap voltage clamp.在凡士林间隙电压钳中测量的青蛙终板乙酰胆碱受体的脱敏作用。
J Physiol. 1989 Aug;415:159-88. doi: 10.1113/jphysiol.1989.sp017717.
4
An analysis of the dose-response relationship at voltage-clamped frog neuromuscular junctions.电压钳制的青蛙神经肌肉接头处剂量-反应关系的分析。
J Physiol. 1978 Aug;281:421-44. doi: 10.1113/jphysiol.1978.sp012431.
5
Desensitization at the frog neuromuscular junction: a biphasic process.青蛙神经肌肉接头处的脱敏作用：一个双相过程。
J Physiol. 1982 Jan;322:257-72. doi: 10.1113/jphysiol.1982.sp014036.
6
Ruthenium red reduces acetylcholine sensitivity and increases desensitization at the frog neuromuscular junction.钌红可降低青蛙神经肌肉接头处的乙酰胆碱敏感性，并增加脱敏作用。
Neuroscience. 1987 Nov;23(2):739-44. doi: 10.1016/0306-4522(87)90091-1.
7
Comparison of cholinergic activation and desensitization at snake twitch and slow muscle fibre end-plates.蛇类快肌和慢肌纤维终板处胆碱能激活与脱敏的比较。
J Physiol. 1984 Jun;351:657-74. doi: 10.1113/jphysiol.1984.sp015269.
8
Desensitization onset and recovery at the potassium-depolarized frog neuromuscular junction are voltage sensitive.钾离子去极化的青蛙神经肌肉接头处脱敏作用的起始和恢复对电压敏感。
J Gen Physiol. 1978 Mar;71(3):285-99. doi: 10.1085/jgp.71.3.285.
9
Fast desensitization of the nicotinic receptor at the mouse neuromuscular junction.小鼠神经肌肉接头处烟碱型受体的快速脱敏
Br J Pharmacol. 1982 Nov;77(3):395-404. doi: 10.1111/j.1476-5381.1982.tb09311.x.
10
Interaction between nerve-related acetylcholine and bath applied agonists at the frog end-plate.蛙终板处神经源性乙酰胆碱与浴槽中应用的激动剂之间的相互作用。
J Physiol. 1980 Feb;299:533-52. doi: 10.1113/jphysiol.1980.sp013141.
引用本文的文献
1
The Structure, Function, and Physiology of the Fetal and Adult Acetylcholine Receptor in Muscle.胎儿及成人肌肉中乙酰胆碱受体的结构、功能与生理学
Front Mol Neurosci. 2020 Sep 8;13:581097. doi: 10.3389/fnmol.2020.581097. eCollection 2020.
2
Rapsyn facilitates recovery from desensitization in fetal and adult acetylcholine receptors expressed in a muscle cell line.Rapsyn 促进了在肌细胞系中表达的胎儿和成人乙酰胆碱受体脱敏后的恢复。
J Physiol. 2019 Jul;597(14):3713-3725. doi: 10.1113/JP277819. Epub 2019 Jun 17.
3
Kinetic evidence that desensitized nAChR may promote transitions of active nAChR to desensitized states during sustained exposure to agonists in skeletal muscle.动力学证据表明，在骨骼肌持续暴露于激动剂期间，脱敏的烟碱型乙酰胆碱受体（nAChR）可能会促进活性nAChR向脱敏状态的转变。
Pflugers Arch. 2006 Jun;452(3):349-62. doi: 10.1007/s00424-006-0043-z. Epub 2006 Mar 23.
4
Acetylcholine receptor channel structure in the resting, open, and desensitized states probed with the substituted-cysteine-accessibility method.用半胱氨酸替代可及性方法探究静息、开放和脱敏状态下的乙酰胆碱受体通道结构。
Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):1241-8. doi: 10.1073/pnas.98.3.1241. Epub 2001 Jan 16.
5
Desensitization of mouse nicotinic acetylcholine receptor channels. A two-gate mechanism.小鼠烟碱型乙酰胆碱受体通道的脱敏。一种双门控机制。
J Gen Physiol. 1998 Aug;112(2):181-97. doi: 10.1085/jgp.112.2.181.
6
Recovery from desensitization of neuronal nicotinic acetylcholine receptors of rat chromaffin cells is modulated by intracellular calcium through distinct second messengers.大鼠嗜铬细胞神经元烟碱型乙酰胆碱受体脱敏后的恢复过程受细胞内钙通过不同第二信使的调节。
J Neurosci. 1998 Apr 1;18(7):2458-66. doi: 10.1523/JNEUROSCI.18-07-02458.1998.
7
Accelerated desensitization of nicotinic receptor channels and its dependence on extracellular calcium in isolated skeletal muscles of streptozotocin-diabetic mice.链脲佐菌素诱导的糖尿病小鼠离体骨骼肌中烟碱受体通道的加速脱敏及其对细胞外钙的依赖性
Br J Pharmacol. 1995 Sep;116(1):1680-4. doi: 10.1111/j.1476-5381.1995.tb16391.x.
8
On the role of G protein activation and phosphorylation in desensitization to acetylcholine in guinea-pig atrial cells.关于G蛋白激活和磷酸化在豚鼠心房细胞对乙酰胆碱脱敏中的作用
J Physiol. 1993 May;464:649-79. doi: 10.1113/jphysiol.1993.sp019656.
9
Postsynaptic nicotinic receptor desensitized by non-contractile Ca2+ mobilization via protein kinase-C activation at the mouse neuromuscular junction.通过蛋白激酶 C 激活在小鼠神经肌肉接头处由非收缩性 Ca2+ 动员引起脱敏的突触后烟碱型受体
Br J Pharmacol. 1995 Jan;114(2):461-7. doi: 10.1111/j.1476-5381.1995.tb13249.x.
10
Comparison of cholinergic activation and desensitization at snake twitch and slow muscle fibre end-plates.蛇类快肌和慢肌纤维终板处胆碱能激活与脱敏的比较。
J Physiol. 1984 Jun;351:657-74. doi: 10.1113/jphysiol.1984.sp015269.
本文引用的文献
1
THE DEPENDENCE OF CONTRACTION AND RELAXATION OF MUSCLE FIBRES FROM THE CRAB MAIA SQUINADO ON THE INTERNAL CONCENTRATION OF FREE CALCIUM IONS.螃蟹（黄道蟹）肌纤维收缩与舒张对游离钙离子胞内浓度的依赖性
Biochim Biophys Acta. 1964 May 25;79:581-91. doi: 10.1016/0926-6577(64)90224-4.
2
Active phase of frog's end-plate potential.青蛙终板电位的活动期
J Neurophysiol. 1959 Jul;22(4):395-411. doi: 10.1152/jn.1959.22.4.395.
3
A study of the desensitization produced by acetylcholine at the motor end-plate.一项关于乙酰胆碱在运动终板产生脱敏作用的研究。
J Physiol. 1957 Aug 29;138(1):63-80. doi: 10.1113/jphysiol.1957.sp005838.
4
A critique of compartmental analysis.隔室分析的批判
Annu Rev Biophys Bioeng. 1981;10:531-62. doi: 10.1146/annurev.bb.10.060181.002531.
5
Desensitization at the frog neuromuscular junction: a biphasic process.青蛙神经肌肉接头处的脱敏作用：一个双相过程。
J Physiol. 1982 Jan;322:257-72. doi: 10.1113/jphysiol.1982.sp014036.
6
Interaction between nerve-related acetylcholine and bath applied agonists at the frog end-plate.蛙终板处神经源性乙酰胆碱与浴槽中应用的激动剂之间的相互作用。
J Physiol. 1980 Feb;299:533-52. doi: 10.1113/jphysiol.1980.sp013141.
7
Desensitization of the acetylcholine receptor of denervated rat soleus muscle and the effect of calcium.去神经大鼠比目鱼肌乙酰胆碱受体的脱敏作用及钙的影响
Br J Pharmacol. 1980 May;69(1):91-8. doi: 10.1111/j.1476-5381.1980.tb10886.x.
8
Activation, inactivation, and desensitization of acetylcholine receptor channel complex detected by binding of perhydrohistrionicotoxin.通过全氢组胺毒素结合检测乙酰胆碱受体通道复合物的激活、失活和脱敏
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2309-13. doi: 10.1073/pnas.77.4.2309.
9
Mechanisms of synaptic modulation.突触调制机制。
Int Rev Neurobiol. 1981;22:205-50. doi: 10.1016/s0074-7742(08)60294-9.
10
Intracellular calcium and desensitization of acetylcholine receptors.细胞内钙与乙酰胆碱受体的脱敏作用
Proc R Soc Lond B Biol Sci. 1980 Sep 26;209(1176):447-52. doi: 10.1098/rspb.1980.0106.
Zotero 插件