Stenbäck F, Wasenius V M, Kallioinen M
J Invest Dermatol. 1986 Aug;87(2):185-9. doi: 10.1111/1523-1747.ep12695326.
Basement membrane changes in the epidermis and hair follicle apparatus resulting from topical 9,10-dimethylbenzanthracene applications were studied in mice, rats, and hamsters by light and electron microscopy and using antibodies to human collagen type IV and laminin. The basement membrane was distinct in epidermal hyperplasia, dysplasia, and papillomas, as well as around most of the keratoacanthomas and squamous cell carcinomas, which showed basement membrane irregularities, thickening, and reduplication in some areas. The invading edges of the squamous cell carcinomas with inflammatory infiltrates were devoid of laminin and collagen. Collagen IV and laminin-positive structures were observed around preserved follicular structures in rat: hair nevi and hair-follicle nevi, but partly absent around trichoepitheliomas and trichofolliculomas. Basal cell tumors were usually surrounded by a distinct basement membrane, which was lacking around some tumor cells.
通过光学显微镜和电子显微镜，并使用抗人IV型胶原蛋白和层粘连蛋白的抗体，研究了局部应用9,10 - 二甲基苯并蒽后小鼠、大鼠和仓鼠表皮和毛囊器官中的基底膜变化。基底膜在表皮增生、发育异常和乳头状瘤中明显可见，在大多数角化棘皮瘤和鳞状细胞癌周围也可见，这些病变在某些区域表现出基底膜不规则、增厚和重复。伴有炎症浸润的鳞状细胞癌的浸润边缘缺乏层粘连蛋白和胶原蛋白。在大鼠保留的毛囊结构（毛发痣和毛囊痣）周围观察到IV型胶原蛋白和层粘连蛋白阳性结构，但在毛发上皮瘤和毛囊瘤周围部分缺失。基底细胞瘤通常被一层明显的基底膜包围，但在一些肿瘤细胞周围则没有。
