Morii T, Nakamura K, Lee Y C, Iijima T, Hoji K
J Protozool. 1986 May;33(2):231-4. doi: 10.1111/j.1550-7408.1986.tb05597.x.
The Taiwanese strain of Leucocytozoon caulleryi was isolated from an infected chicken in Taipei, Taiwan, and established in chickens and biting midges Culicoides arakawae from Japan. Sporogony of the strain in C. arakawae was completed on day 3 after the infective blood meals at 25 degrees C. Sporozoites isolated from the salivary glands of C. arakawae on days 3 or 4 after feeding caused infection in all the chickens inoculated. The strain showed high pathogenicity for chickens. Mortality of chickens rose with an increase in the number of sporozoites inoculated. The prepatent period for chickens inoculated with sporozoites was 14 days. Parasites appeared in the peripheral blood of chickens on day 15 and disappeared on day 26 after sporozoite inoculation. Soluble antigens were found in the sera of chickens infected with the strain between 10 and 17 days after inoculation, and homologous antibodies appeared after 17 days. Antigens prepared from sera, schizonts, merozoites, and gametocytes of the Taiwanese strain reacted with the sera of chickens infected with the same strain or the strain isolated in Japan. The chickens that recovered from a primary infection with the Taiwanese strain demonstrated complete resistance to reinfection with the same strain or the the strain isolated in Japan.
台湾卡氏住白细胞虫株是从台湾台北一只受感染的鸡中分离出来的，并在来自日本的鸡和蠛蠓（Culicoides arakawae）中传代建立。该虫株在25摄氏度下，于感染血餐3天后在蠛蠓中完成孢子生殖。在喂食后第3天或第4天从蠛蠓唾液腺分离出的子孢子感染了所有接种的鸡。该虫株对鸡表现出高致病性。鸡的死亡率随着接种子孢子数量的增加而上升。接种子孢子的鸡的潜伏期为14天。接种子孢子后第15天鸡外周血中出现寄生虫，第26天消失。在接种该虫株10至17天后感染鸡的血清中发现可溶性抗原，17天后出现同源抗体。从台湾虫株的血清、裂殖体、裂殖子和配子体制备的抗原与感染同一虫株或日本分离株的鸡的血清发生反应。从台湾虫株初次感染中康复的鸡对同一虫株或日本分离株的再次感染表现出完全抗性。
