Moril T, Matsui T, Iijima T, Abe N, Yoshizawa A, Chikatsune M, Watanabe M
Department of Parasitology, Kyorin University School of Medicine, Tokyo, Japan.
J Protozool. 1988 Aug;35(3):356-9. doi: 10.1111/j.1550-7408.1988.tb04106.x.
Leucocytozoon caulleryi sporozoites that had been stored at -196 degrees C or -80 degrees C for 6 or 12 months in Eagle's minimum essential medium or Medium 199 supplemented with 5% glycerol and 10% chicken serum showed infectivity to chickens. Glycerol at a concentration of 10% and dimethyl sulfoxide at 10% and 5% were found to be ineffective cryoprotective agents for the low temperature preservation of sporozoites. Sporozoites isolated from the intact females of Culicoides arakawae, which had been stored at -80 degrees C for 6 or 12 months without cryoprotective agents, retained their infectivity. No differences were observed in the prepatent period, duration of parasitemia, and presence of serum-soluble antigens between chickens infected with frozen sporozoites and those infected with fresh sporozoites.
卡氏住白细胞虫子孢子在添加5%甘油和10%鸡血清的伊格尔氏最低必需培养基或199培养基中于-196℃或-80℃保存6个月或12个月后，对鸡仍具有感染力。发现浓度为10%的甘油以及10%和5%的二甲亚砜作为子孢子低温保存的冷冻保护剂无效。从无冷冻保护剂在-80℃保存6个月或12个月的荒川库蠓完整雌虫中分离出的子孢子仍具有感染力。感染冷冻子孢子的鸡和感染新鲜子孢子的鸡在潜隐期、虫血症持续时间以及血清可溶性抗原的存在方面未观察到差异。
