Morii T, Matsui T, Fujino T, Kobayashi F, Tsuji T
Department of Tropical Diseases and Parasitology, Kyorin University School of Medicine, Tokyo, Japan.
Parasitol Res. 1996;82(5):454-8. doi: 10.1007/s004360050144.
The immunogenicity of Leucocytozoon caulleryi sporozoites for chickens and their reactivity in vitro with specific immune sera were studied. Almost all of the chickens that had been immunized with the sporozoite antigens survived the sporozoite challenge. The degree of parasitemia observed in the immunized chickens was significantly lower than that found in the nonimmunized chickens. Specific antibodies against sporozoites were tested by the circumsporozoite precipitation (CSP) reaction. Antibodies were demonstrated in the sera of chickens that had been immunized with the sporozoite antigens or chickens that had recovered from a primary infection with L. caulleryi sporozoites. When viable mature sporozoites were incubated in vitro with serum from immune chickens, agglutination and a long, thread-like precipitate at one end of the sporozoite could be seen within a few minutes under a phase-contrast microscope. The effects of specific immune serum on the infectivity of sporozoites were examined by the sporozoite neutralization activity (SNA) test. Sporozoites that had been incubated in vitro with serum from immune chickens lost their infectivity to chickens. The CSP reaction and the SNA test in L. caulleryi infection were stage- and species-specific.
研究了卡氏住白细胞虫子孢子对鸡的免疫原性及其在体外与特异性免疫血清的反应性。几乎所有用子孢子抗原免疫的鸡在受到子孢子攻击后都存活下来。免疫鸡中观察到的虫血症程度明显低于未免疫鸡。通过环子孢子沉淀（CSP）反应检测针对子孢子的特异性抗体。在用子孢子抗原免疫的鸡或从初次感染卡氏住白细胞虫子孢子中恢复的鸡的血清中都检测到了抗体。当将活的成熟子孢子与免疫鸡的血清在体外孵育时，在相差显微镜下几分钟内就可以看到子孢子一端出现凝集和长丝状沉淀。通过子孢子中和活性（SNA）试验检测特异性免疫血清对子孢子感染力的影响。与免疫鸡血清在体外孵育的子孢子对鸡失去了感染力。卡氏住白细胞虫感染中的CSP反应和SNA试验具有阶段特异性和种特异性。
