Hughes Summer R, Chapleau Richard R
1st American Systems and Services, Falls Church, VA, USA.
Applied Technology & Genomics Division, Aeromedical Research Department, U.S. Air Force School of Aerospace Medicine, 711th Human Performance Wing, Air Force Research Laboratory, 2510 Fifth St, Dayton, Wright-Patterson AFB, OH, 45433, USA.
BMC Res Notes. 2019 Mar 23;12(1):165. doi: 10.1186/s13104-019-4211-6.
With the democratization of genetic testing, researchers, clinicians, and educators must consider the varying degree of field conditions when collecting samples for genetic analyses. For genotyping or sequencing studies, study designers have multiple options from which to choose, including cheek swabs and saliva sampling. One significant benefit of saliva collection is that it can be done remotely, in the privacy of one's home. This same benefit adds a risk of compliance. Therefore, our goal with this study was to see if the quality and quantity of the saliva collection by a saliva DNA collection kit would be affected by not following the manufacturer's directions, i.e., drinking or eating right before collection.
We asked five participants to collect saliva samples according to the manufacturer's guidance and also after consuming five food items or beverages. We evaluated DNA quantity and quality post-purification using spectroscopy, electrophoresis, and polymerase chain reaction genotyping. Consistent with our hypothesis, we did not see a difference in quantity or quality of the isolated DNA. From our results, we conclude that the manufacturer's instructions serve as an ideal guideline, but the collection devices are robust enough to permit flexibility in sampling at home or in the field.
随着基因检测的普及,研究人员、临床医生和教育工作者在为基因分析收集样本时必须考虑不同程度的现场条件。对于基因分型或测序研究,研究设计者有多种选择,包括颊拭子和唾液采样。唾液采集的一个显著优点是可以在自己家中私下远程进行。同样的优点也带来了合规风险。因此,我们这项研究的目的是查看唾液DNA采集试剂盒收集的唾液质量和数量是否会受到不遵循制造商说明(即在采集前立即饮食)的影响。
我们让五名参与者按照制造商的指导以及在食用五种食品或饮料后收集唾液样本。我们使用光谱学、电泳和聚合酶链反应基因分型评估纯化后的DNA数量和质量。与我们的假设一致,我们没有看到分离出的DNA在数量或质量上有差异。根据我们的结果,我们得出结论,制造商的说明是理想的指导方针,但采集设备足够坚固,允许在家中或现场采样时具有灵活性。