Hamed Houda, Bellassoued Khaled, El Feki Abdelfattah, Gargouri Ahmed
1Laboratory of Animal Ecophysiology, Faculty of Sciences, Sfax University, BP 3038, Sfax, Tunisia.
2Research Unit of Toxicology, Environmental Microbiology and Health, Faculty of Science of Sfax, University of Sfax, 3064 Sfax, Tunisia.
J Food Sci Technol. 2019 Feb;56(2):824-834. doi: 10.1007/s13197-018-3542-y. Epub 2019 Jan 5.
The present study was conducted to evaluate the in vitro antioxidant activity of fermented camel milk with subsp. (FCM-LLC) alone, extract (R) alone and their combination and to investigate their hepatopreventive effects against CCl liver damage in mice. The antioxidant activity in vitro of FMC-LLC supplemented with R exhibited the highest free radical scavenging and ferric reducing power activities. The results showed that the pretreatment with a combination of FMC-LLC and R significantly alleviated the increased levels of hepatic markers and the elevated lipid levels induced by CCl in mice. Meanwhile, the enzymatic antioxidants activities (superoxide dismutase, glutathione peroxidase, and catalase) and GSH level in liver significantly were increased while the malondialdehyde level was significantly improved by pretreatment with FMLLC plus R. These data suggest that FCM-LLC in combination with extract possesses better antioxidant and hepatoprotective activity than FMC-LLC alone.
本研究旨在评估单独的发酵骆驼奶亚种（FCM-LLC）、单独的提取物（R）及其组合的体外抗氧化活性，并研究它们对小鼠四氯化碳肝损伤的肝保护作用。添加R的FMC-LLC的体外抗氧化活性表现出最高的自由基清除和铁还原能力活性。结果表明，FMC-LLC和R联合预处理显著减轻了四氯化碳诱导的小鼠肝脏标志物水平升高和脂质水平升高。同时，肝脏中的酶促抗氧化剂活性（超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶）和谷胱甘肽水平通过FMLLC加R预处理显著增加，而丙二醛水平显著改善。这些数据表明，FCM-LLC与提取物组合比单独的FMC-LLC具有更好的抗氧化和肝保护活性。
