莲荷叶的体外抗氧化活性及体内保肝作用
Antioxidant activities in vitro and hepatoprotective effects of Nelumbo nucifera leaves in vivo.
作者信息
Yuan Lin, Gu Xuezhu, Yin Zhenhua, Kang Wenyi
机构信息
Institute of Chinese Materia, Henan University, Kaifeng 475004, China ; Chemical Science and Technology Department, Kunming University, Yunnan Kunming 650214, China.
Institute of Chinese Materia Medica, Traditional Chinese Medical Research Institute, Beijing, 100700, China.
出版信息
Afr J Tradit Complement Altern Med. 2014 Apr 3;11(3):85-91. doi: 10.4314/ajtcam.v11i3.12. eCollection 2014.
BACKGROUND
Herbal medicines played a major role in the treatment of hepatic disorders, and a number of medicinal plants and their compounds were widely used for the treatment of these disorders, and oxidant stress injury was one of the mechanism of liver injury.
MATERIALS AND METHODS
Antioxidant activity of Nelumbo nucifera leaves (NU) extracts was assayed by the methods of scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzo-thiazoline-6-sulfonicacid) (ABTS) radical and ferric reducing antioxidant power (FRAP) in vitro. By intraperitoneal injection carbon tetrachloride (CCl4) to establish acute liver injury model in mice, the levels of Glutamic-pyruvic transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), superoxide dismutase (SOD) and the content of and maleicdialdehyde (MDA) were detected to evaluate hepatoprotective effect of NU using corresponding test kit.
RESULTS
EtOAC (NUEA) and n-BuOH extracts (NUBU) of N. nucifera leaves had good scavenging DPPH and ABTS radical activity and ferric reducing antioxidant power in vitro. DPPH radical scavenging activity and ferric reducing antioxidant power of NUEA (IC50= 6.68±0.29 µg/mL, RACT50=1749.82±67.03 µmol/g) and NUBU (IC50= 4.61±0.01 µg/mL, RACT50=1995.27±135.71 µmol/g ) were higher than that of BHT (IC50=8.76±0.20 µg/mL, RACT50=1581.68±97.41 µmol/g) and Dangfeiliganning (IC50=28.06±0.17 µg/mL, RACT50=1028.55±3.28 µmol/g). ABTS radical scavenging activity of NUEA (IC50= 5.32±0.12 µg/mL) and NUBU (IC50= 8.16±0.27 µg/mL) were higher than that of Dangfeiliganning (IC50= 9.76±0.16 µg/mL). Thus, hepatoprotective effect of NUEA and NUBU was evaluated on CCl4-induced acute liver injury mice. The results showed that the levels of GOT and GPT in each treatment group significantly decreased (p<0.001 and p<0.01, p<0.05, respectively) except for the group of NUEA (130.8 mg/kg) (p>0.05). The contents of malondialdehyde (MDA) in liver in groups of NUEA (523 mg/kg), NUBU (840.5 and 420.5 mg/kg, repectively) had significant decrease (p<0.001 and p<0.05, respectively), and the level of SOD in liver for each treatment group could significantly decrease (p<0.001, p<0.05, respectively).
CONCLUSION
NUEA and NUBU had significantly hepatoprotective effect for Calcium tetrachloride CCl4-induced liver injury, which might be attributable to its antioxidant activity.
背景
草药在肝脏疾病的治疗中发挥着重要作用，许多药用植物及其化合物被广泛用于治疗这些疾病，氧化应激损伤是肝损伤的机制之一。
材料与方法
采用体外清除1,1-二苯基-2-苦基肼（DPPH）、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基及铁离子还原抗氧化能力（FRAP）的方法，检测莲（NU）叶提取物的抗氧化活性。通过腹腔注射四氯化碳（CCl4）建立小鼠急性肝损伤模型，使用相应试剂盒检测谷丙转氨酶（GPT）、谷草转氨酶（GOT）、超氧化物歧化酶（SOD）水平及丙二醛（MDA）含量，以评价NU的保肝作用。
结果
莲（NU）叶的乙酸乙酯提取物（NUEA）和正丁醇提取物（NUBU）在体外具有良好的清除DPPH和ABTS自由基活性及铁离子还原抗氧化能力。NUEA（IC50 = 6.68±0.29 μg/mL，RACT50 = 1749.82±67.03 μmol/g）和NUBU（IC50 = 4.61±0.01 μg/mL，RACT50 = 1995.27±135.71 μmol/g）的DPPH自由基清除活性和铁离子还原抗氧化能力高于二丁基羟基甲苯（BHT）（IC50 = 8.76±0.20 μg/mL，RACT50 = 1581.68±97.41 μmol/g）和当飞利肝宁（IC50 = 28.06±0.17 μg/mL，RACT50 = 1028.55±3.28 μmol/g）。NUEA（IC50 = 5.32±0.12 μg/mL）和NUBU（IC50 = 8.16±0.27 μg/mL）的ABTS自由基清除活性高于当飞利肝宁（IC50 = 9.76±0.16 μg/mL）。因此，在CCl4诱导的急性肝损伤小鼠上评价了NUEA和NUBU的保肝作用。结果显示，除NUEA（130.8 mg/kg）组外（p>0.05），各治疗组的GOT和GPT水平均显著降低（分别为p<0.001、p<0.01、p<0.05）。NUEA（523 mg/kg）组、NUBU（分别为840.5和420.5 mg/kg）组肝脏中丙二醛（MDA）含量显著降低（分别为p<0.001和p<0.05），各治疗组肝脏中SOD水平也显著降低（分别为p<0.001、p<0.05）。
结论
NUEA和NUBU对四氯化碳（CCl4）诱导的肝损伤具有显著的保肝作用，这可能与其抗氧化活性有关。
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