Conti Juan P, Vinderola Gabriel, Esteban Eduardo N
1Laboratorio de Virología, Centro de Investigación Veterinaria de Tandil (CIVETAN, CONICET-CICPBA), Facultad de Cs. Veterinarias, UNCPBA, Pasaje Arroyo Seco s/n, 7000 Tandil, Argentina.
2Instituto de Lactología Industrial (INLAIN, UNL-CONICET), Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santiago del Estero 2829, 3000 Santa Fe, Argentina.
J Food Sci Technol. 2019 Feb;56(2):896-904. doi: 10.1007/s13197-018-3551-x. Epub 2019 Jan 1.
Argentina is a leading country in biodiesel production from soy. Extruded soy is a low-cost byproduct of the soybean oil industry, from which animal feeds are prepared as well as flour for human consumption. Soy proteins can be isolated from flours and digested with enzymes in order to obtain bioactive fractions. In this work, a commercial soy isolate (PRO-FAM 974) was characterized. Maximal solubility was achieved at a concentration of 90 mg/mL. Protein profiles obtained by SDS-PAGE showed that the isolate was constituted mostly by globulins. Conformational and thermal analyses (differential scanning calorimetry) showed that proteins were almost completely denatured. The isolate was hydrolyzed with a commercially available enzyme (COROLASE 7089). The peptide profile (MALDI-TOF) showed peptides ranging from 800 to 10,000 Daltons. We conclude that the product obtained has the potential to be used as functional ingredient for the development of functional foodstuffs, giving the opportunity to add value to the byproducts of the soybean oil industry.
阿根廷是大豆生物柴油生产的主要国家。挤压大豆是大豆油行业的一种低成本副产品，可从中制备动物饲料以及供人类食用的面粉。大豆蛋白可从面粉中分离出来，并用酶进行消化，以获得生物活性成分。在这项工作中，对一种市售大豆分离蛋白（PRO-FAM 974）进行了表征。在浓度为90毫克/毫升时达到最大溶解度。通过SDS-PAGE获得的蛋白质图谱表明，该分离蛋白主要由球蛋白组成。构象和热分析（差示扫描量热法）表明蛋白质几乎完全变性。该分离蛋白用市售酶（COROLASE 7089）进行水解。肽谱（基质辅助激光解吸电离飞行时间质谱）显示肽的分子量范围为800至10000道尔顿。我们得出结论，所获得的产品有潜力用作功能性食品开发的功能成分，为大豆油行业的副产品增加价值提供了机会。
