Erkiş-Güngör Gözde, Çevik Bayram
Applied Sciences University of Isparta, Faculty of Agricultural Sciences and Technologies, Department of Plant Protection, 32260 Isparta, Turkey.
Ministry of Food, Agriculture and Livestock, Antalya Agricultural Quarantine Office, Virology Laboratory, 07260 Antalya, Turkey.
Adv Virol. 2019 Jan 1;2019:7163747. doi: 10.1155/2019/7163747. eCollection 2019.
The presence of (CTV) in Turkey has been known since the 1960s and the virus was detected in all citrus growing regions of the country. Even though serological and biological characteristics of CTV have been studied since the 1980s, molecular characteristics of CTV isolates have not been studied to date in Turkey. In this study, molecular characteristics of 15 CTV isolates collected from different citrus growing regions of Turkey were determined by amplification, cloning, and sequencing of their major coat protein (CP) genes. The sequence analysis showed that the CP genes were highly conserved among Turkish isolates. However, isolates from different regions showed more genetic variation than isolates from the same region. Turkish isolates were clustered into three phylogenetic groups showing no association with geographical origins, host, or symptoms induced in indicator plants. Phylogenetic analysis of Turkish isolates with isolates from different citrus growing regions of the world including well-characterized type isolates of previously established strain specific groups revealed that some Turkish isolates were closely related to severe quick decline or stem pitting isolates. The results demonstrated that although CTV isolates from Turkey are considered biologically mild, majority of them contain severe components potentially causing quick decline or stem pitting.
自20世纪60年代以来，土耳其就已发现柑橘衰退病毒（CTV），该国所有柑橘种植区都检测到了这种病毒。尽管自20世纪80年代以来就对CTV的血清学和生物学特性进行了研究，但迄今为止，土耳其尚未对CTV分离株的分子特性进行研究。在本研究中，通过对来自土耳其不同柑橘种植区的15个CTV分离株的主要外壳蛋白（CP）基因进行扩增、克隆和测序，确定了其分子特性。序列分析表明，土耳其分离株的CP基因高度保守。然而，来自不同地区的分离株比来自同一地区的分离株表现出更多的遗传变异。土耳其分离株被聚类为三个系统发育组，与地理来源、宿主或指示植物中诱导的症状无关。对土耳其分离株与来自世界不同柑橘种植区的分离株进行系统发育分析，包括先前已确定的菌株特异性组的特征明确的类型分离株，结果显示一些土耳其分离株与严重快速衰退或茎陷点分离株密切相关。结果表明，尽管来自土耳其的CTV分离株在生物学上被认为是温和的，但它们中的大多数含有可能导致快速衰退或茎陷点的严重成分。
