Brlansky R H, Damsteegt V D, Howd D S, Roy A
Professor, University of Florida, Citrus Research and Education Center, Lake Alfred.
Reasearch Plant Pathologist, USDA, ARS, FDWSRU, Fort Detrick, Frederick, MD.
Plant Dis. 2003 Apr;87(4):397-401. doi: 10.1094/PDIS.2003.87.4.397.
Citrus tristeza virus (CTV) exists in field isolates as a complex of virus isolates. This complex may contain both mild and severe CTV. Using single and multiple aphid transmissions, subiso-lates of the various field isolates were separated. Some CTV isolates that tested negative with the monoclonal antibody MCA13 consisted of MCA13-positive subisolates. Using primers to specific and variable regions of the CTV genome, molecular profiles of the isolates and subisolates were generated and compared. The profiles of the subisolates sometimes were very different from the parent field isolates from which they were transmitted.
柑橘衰退病毒（CTV）在田间分离株中以病毒分离株复合体的形式存在。该复合体可能同时包含温和型和强毒株型的CTV。通过单头和多头蚜虫传毒，分离出了各种田间分离株的亚分离株。一些用单克隆抗体MCA13检测呈阴性的CTV分离株由MCA13阳性的亚分离株组成。使用针对CTV基因组特定区域和可变区域的引物，生成并比较了分离株和亚分离株的分子图谱。亚分离株的图谱有时与它们所传自的亲本田间分离株有很大不同。
