Zhang Wenhao, Sun Jiakui, Shen Xiao, Xue Yinying, Yuan Shoutao, Wang Xiang
Department of Critical Care Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China.
Exp Ther Med. 2019 Apr;17(4):2567-2574. doi: 10.3892/etm.2019.7247. Epub 2019 Feb 6.
Effects of blank -mannose sensitive hemagglutini (PA-MSHA) preprocessing on toll like receptor (TLR)-4-NF-κB pathway and inflammatory factors expression in the intestinal tract of rats with septic shock were investigated. A total of 30 rats were randomly divided into 3 groups (n=10): Blank control, septic shock, and PA-septic shock group. After the model was successfully established, the average arterial pressure in rats was monitored. The concentration of cytokine interleukin-l (IL-1), IL-6 and tumor necrosis factor-α (TNF-α) were determined by ELISA method. Flow cytometry was performed to detect TLR-4 expression. Number of chemotaxised neutrophils was detected by Transwell chamber. The expression of TLR-4, NF-κB and ICAM-1 and VCAM-1 was detected by western blot analysis. The concentration of cytokine IL-1, IL-6, TNF-α in the peritoneal lavage fluid and the intestinal tissue significantly increased in the septic shock group and the PA-septic shock group (P<0.05), and the concentration in the PA-septic shock group was significantly lower than that of the septic shock group (P<0.05). Compared to the control group, the expression of TLR-4, NF-κB and ICAM-1 and VCAM-1 increased in the septic shock and PA-septic shock group (P<0.05), and the expression level of PA-septic shock group was lower than the septic shock group (P<0.05). The expression of TLR-4 in the PA-septic shock group was lower than the septic shock group (P<0.05). PA-MSHA pretreatment reduced inflammation, thus preventing the intestinal injury caused by septic shock.
研究空白甘露糖敏感血凝素（PA-MSHA）预处理对脓毒症休克大鼠肠道中Toll样受体（TLR）-4-NF-κB信号通路及炎症因子表达的影响。将30只大鼠随机分为3组（n = 10）：空白对照组、脓毒症休克组和PA-脓毒症休克组。成功建立模型后，监测大鼠平均动脉压。采用酶联免疫吸附测定（ELISA）法测定细胞因子白细胞介素-1（IL-1）、IL-6和肿瘤坏死因子-α（TNF-α）的浓度。采用流式细胞术检测TLR-4表达。通过Transwell小室检测趋化的中性粒细胞数量。采用蛋白质免疫印迹法检测TLR-4、NF-κB、细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的表达。脓毒症休克组和PA-脓毒症休克组腹腔灌洗液和肠组织中细胞因子IL-（1）、IL-6、TNF-α的浓度显著升高（P < 0.05），且PA-脓毒症休克组浓度显著低于脓毒症休克组（P < 0.05）。与对照组相比，脓毒症休克组和PA-脓毒症休克组TLR-4、NF-κB、ICAM-1和VCAM-1表达增加（P < 0.05），且PA-脓毒症休克组表达水平低于脓毒症休克组（P < 0.05）。PA-脓毒症休克组TLR-4表达低于脓毒症休克组（P < 0.05）。PA-MSHA预处理减轻炎症反应，从而预防脓毒症休克所致的肠道损伤。
