Saito Kazuki, Suzuki Rie, Koyanagi Yukako, Isogai Hiroshi, Yoneyama Hiroshi, Isogai Emiko
Department of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8577, Japan.
Animal Research Center, Sapporo Medical University, Sapporo, Hokkaido 060-8556, Japan.
Biomed Rep. 2019 Mar;10(3):175-182. doi: 10.3892/br.2019.1193. Epub 2019 Feb 7.
Enterohemorrhagic (EHEC) O157:H7 has been known to cause outbreaks of hemorrhagic colitis and hemolytic uremic syndrome. We previously demonstrated that intestinal flora contribute to the prevention of EHEC infection in a mouse model. However, it has not yet been determined whether , a predominant genus in the human intestine, contributes to the prevention of EHEC infection. The aim of the present study was to investigate the effect of () and (B. vulgatus) on EHEC O157:H7 infection using gnotobiotic mice. These strains were inoculated into germ-free mice to create a gnotobiotic mouse model. EHEC was inoculated into the mice, which were then monitored for 7 days for any change in symptoms. The mice that had been pre-colonized with the strains did not develop lethal EHEC infection, although several inflammatory symptoms were observed in the pre-colonized group. However, no inflammatory symptoms were identified in the pre-colonized group. Moreover, exerted an inhibitory effect on enterocyte-like cell apoptosis. protected HT29 cells from apoptosis caused by Shiga toxin. In conclusion, the findings of the present study demonstrated that colonization by strains can inhibit EHEC infection.
肠出血性大肠杆菌（EHEC）O157:H7可引发出血性结肠炎和溶血尿毒综合征疫情。我们之前在小鼠模型中证明肠道菌群有助于预防EHEC感染。然而，尚未确定人类肠道中的主要菌属——是否有助于预防EHEC感染。本研究的目的是使用无菌小鼠研究（）和普通拟杆菌（B. vulgatus）对EHEC O157:H7感染的影响。将这些菌株接种到无菌小鼠体内以建立无菌小鼠模型。将EHEC接种到小鼠体内，然后监测7天以观察症状的任何变化。预先定殖有菌株的小鼠未发生致命的EHEC感染，尽管在预先定殖组中观察到了一些炎症症状。然而，在预先定殖组中未发现炎症症状。此外，对肠上皮样细胞凋亡具有抑制作用。保护HT29细胞免受志贺毒素引起的凋亡。总之，本研究结果表明菌株定殖可抑制EHEC感染。
