Sharma A, Kumar Y, Kumar G, Tahlan A K
National Salmonella and Escherichia Centre, Central Research Institute, Kasauli, Himachal Pradesh 173204 India.
Indian J Microbiol. 2024 Dec;64(4):1512-1517. doi: 10.1007/s12088-023-01149-7. Epub 2023 Dec 9.
and are important enteric pathogens associated with a variety of infections. Biofilm formation and antimicrobial resistance are important characteristics making these pathogens a concern in terms of strong attachment to substrates, expression of virulence markers and difficult removal. The present study investigates the biofilm-forming ability and antibiogram patterns among and . A total of 200 . and 100 isolates received at National Salmonella and Escherichia Centre were identified by biochemical testing, followed by serotyping. Biofilm production was detected by Tissue Culture Plate method. The isolates were further subjected to Antibiotic Susceptibility Testing by the Kirby-Bauer disk diffusion method. 113 (56.5%) . isolates and 79 (79%) isolates were detected as biofilm producers. A total of 114(57%). isolates and 31(31%) isolates were found to be resistant to multiple drugs when Antibiotic Susceptibility Testing was carried out. Antibiotic resistance was found to be significantly higher in biofilm producing salmonella ( = ) whereas in the case of the difference remained non-significant ( = ). The capability to produce biofilm along with acquiring high level of antimicrobial resistance in salmonella and provide enhanced survival potential in adverse environments. Therefore, it becomes a serious cause of concern for public health authorities considering the virulence of these bacteria and their association with different disease conditions and requires urgent intervention with regards to control and prevention strategies.
[未提及的两种病原体]和[另一种未提及的病原体]是与多种感染相关的重要肠道病原体。生物膜形成和抗菌耐药性是这些病原体的重要特征,使得它们因对底物的强烈附着、毒力标志物的表达以及难以清除而备受关注。本研究调查了[未提及的两种病原体]和[另一种未提及的病原体]的生物膜形成能力和抗菌谱模式。国家沙门氏菌和大肠杆菌中心共接收了200株[未提及的两种病原体]和100株[另一种未提及的病原体]分离株,通过生化测试进行鉴定,随后进行血清分型。采用组织培养板法检测生物膜产生情况。通过 Kirby-Bauer 纸片扩散法对分离株进一步进行抗生素敏感性测试。113株(56.5%)[未提及的两种病原体]分离株和79株(79%)[另一种未提及的病原体]分离株被检测为生物膜产生菌。进行抗生素敏感性测试时,共发现114株(57%)[未提及的两种病原体]分离株和31株(31%)[另一种未提及的病原体]分离株对多种药物耐药。发现生物膜产生型沙门氏菌的抗生素耐药性显著更高(P值= [具体值未给出]),而对于[另一种未提及的病原体],差异不显著(P值= [具体值未给出])。沙门氏菌和[另一种未提及的病原体]产生生物膜的能力以及获得高水平抗菌耐药性的能力,使其在不利环境中具有更强的生存潜力。因此,考虑到这些细菌的毒力及其与不同疾病状况的关联,这成为公共卫生当局严重关切的问题,需要在控制和预防策略方面进行紧急干预。