Niederhuber J E, Dugan E S, Shaprio L H, Ernst L
Surgery. 1986 Aug;100(2):424-9.
The immune response to the polysaccharide antigen Trinitrophenyl (TNP)-ficoll is controlled by two complementing loci in the murine major histocompatibility complex. One locus of control is present in the I-A subregion and the second is located between the S and D regions. A monoclonal antibody 48-21.7 was selected for its ability to significantly block the in vitro primary response to TNP-ficoll. This antibody bound to antigen-presenting cells does not interfere with the presentation of other antigens. Experiments are presented to demonstrate that the monoclonal antibody 48-21.7 precipitates a unique protein of approximately 40,000 daltons. Sequential immunoprecipitation with monoclonal antibodies to the class I Db antigen and to relevant Ia antigens demonstrated no cross-reactivity. Amino acid sequencing has found unique amino acids to be present at three class I N-terminal invariant positions. These observations indicate the presence of a unique class I-like molecule coded for by genes mapping between S and D and involved in the response to the polysaccharide ficoll.
对多糖抗原三硝基苯基（TNP）- 聚蔗糖的免疫反应由小鼠主要组织相容性复合体中的两个互补基因座控制。一个控制基因座存在于I - A亚区，另一个位于S区和D区之间。一种单克隆抗体48 - 21.7因其能够显著阻断体外对TNP - 聚蔗糖的初次反应而被选中。这种与抗原呈递细胞结合的抗体不会干扰其他抗原的呈递。本文展示的实验表明，单克隆抗体48 - 21.7沉淀出一种独特的约40,000道尔顿的蛋白质。用针对I类Db抗原和相关Ia抗原的单克隆抗体进行的连续免疫沉淀显示没有交叉反应性。氨基酸测序发现在I类N端三个恒定位置存在独特的氨基酸。这些观察结果表明存在一种独特的类I样分子，由位于S区和D区之间的基因编码，并参与对多糖聚蔗糖的反应。
