建立和验证 QuEChERS-LC-MS/MS 法分析菜籽油中的酚类化合物
Development and Validation of a QuEChERS-LC-MS/MS Method for the Analysis of Phenolic Compounds in Rapeseed Oil.
机构信息
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, National Engineering Laboratory for Cereal Fermentation Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province , Jiangnan University , 1800 Lihu Road , Wuxi , Jiangsu 214122 , People's Republic of China.
出版信息
J Agric Food Chem. 2019 Apr 10;67(14):4105-4112. doi: 10.1021/acs.jafc.9b00029. Epub 2019 Apr 1.
In recent years, the determination of phenolic compounds in vegetable oil has aroused broad attention because these compounds have beneficial effects on health. In this work, a novel method based on the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and LC-MS/MS was developed for the analysis of phenolic compounds. A total of 18 mL of acetonitrile, 3 mL of water, and 270 mg of C sorbent were utilized in the optimized QuEChERS procedure. The LC-MS/MS analysis was performed in a C column under gradient-elution conditions with eluent of acetonitrile and water with 0.1% acetic acid. The QuEChERS approach achieved decent extraction recoveries (75.32-103.93%) for most phenolic compounds. The QuEChERS-LC-MS/MS method was validated in terms of accuracy, precision, sensitivity, and linearity. The proposed method was further evaluated using different prepared rapeseed oils. The result demonstrated that QuEChERS-LC-MS/MS is a rapid and reliable method for determining phenolic compounds in rapeseed oils.
近年来，植物油中酚类化合物的测定引起了广泛关注，因为这些化合物对健康有益。本工作建立了一种基于 QuEChERS 方法和 LC-MS/MS 的测定酚类化合物的新方法。在优化的 QuEChERS 方法中，使用了 18 mL 乙腈、3 mL 水和 270 mg C 吸附剂。LC-MS/MS 分析在 C 柱上进行，采用梯度洗脱条件，洗脱剂为乙腈和水，含 0.1%乙酸。QuEChERS 方法对大多数酚类化合物的提取回收率良好（75.32%-103.93%）。对 QuEChERS-LC-MS/MS 方法进行了准确度、精密度、灵敏度和线性度的验证。该方法进一步用于不同制备的菜籽油的评估。结果表明，QuEChERS-LC-MS/MS 是一种快速可靠的测定菜籽油中酚类化合物的方法。
Zotero 插件