The Athinoula A. Martinos Center for Biomedical Imaging, The Institute for Innovation in Imaging, Department of Radiology , Massachusetts General Hospital (MGH) & Harvard Medical School (HMS) , Charlestown , Massachusetts 02129 , United States.
Division of Thoracic Surgery , MGH & HMS , Boston , Massachusetts 02114 , United States.
J Am Chem Soc. 2019 Apr 10;141(14):5593-5596. doi: 10.1021/jacs.8b12342. Epub 2019 Mar 27.
Oxidized collagen, wherein lysine residues are converted to the aldehyde allysine, is a universal feature of fibrogenesis, i.e. actively progressive fibrosis. Here we report the small molecule, allysine-binding positron emission tomography probe, Ga-NODAGA-indole, that can noninvasively detect and quantify pulmonary fibrogenesis. We demonstrate that the uptake of Ga-NODAGA-indole in actively fibrotic lungs is 7-fold higher than in control groups and that uptake is linearly correlated ( R = 0.98) with the concentration of lung allysine.
氧化胶原蛋白，其中赖氨酸残基转化为醛基丙烯醛，是纤维发生的普遍特征，即活跃进行的纤维化。在这里，我们报告了小分子丙烯醛结合正电子发射断层扫描探针 Ga-NODAGA-吲哚，它可以非侵入性地检测和定量肺纤维化。我们证明，在活跃纤维化的肺中，Ga-NODAGA-吲哚的摄取量比对照组高 7 倍，并且摄取量与肺丙烯醛的浓度呈线性相关（R = 0.98）。
