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植物内生真菌感染后的挥发性成分分析。

Volatile profile of plants after endophyte infection.

机构信息

Department of Biology, University of Florence, Sesto Fiorentino, Italy.

Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.

出版信息

Nat Prod Res. 2020 Aug;34(15):2232-2237. doi: 10.1080/14786419.2019.1579810. Epub 2019 Mar 25.

Abstract

The differences in volatile profile of plants not-inoculated (EpC) and inoculated with their endophytes from roots (EpR) and stem/leaves (EpS/L) were analysed and compared by GC-FID/GC-MS in an model. Non-terpenes and sesquiterpene hydrocarbons were the most abundant classes with an opposite behaviour of EpS/L showing a decreased emission of sesquiterpenes and an increase of non-terpene derivatives. The main compounds obtained from EpS/L were ()-8-dodecen-1-ol and 1-pentadecene, while germacrene D and β-caryophyllene were the key compounds in EpC and EpR. For the first time, this work indicates that bacterial endophytes modify the aroma profiles of infected and non-infected plants of the important medicinal plant . Therefore, our model of infection could permit to select endophytic strains to use as biotechnological tool in the production of medicinal plants enriched in volatile bioactive compounds.

摘要

采用 GC-FID/GC-MS 对模型中未接种(EpC)和接种内生菌(EpR 和 EpS/L)的根、茎/叶植物的挥发性成分进行分析和比较。非萜类和倍半萜烃是含量最丰富的两类化合物,其中 EpS/L 的表现出相反的行为,即减少了倍半萜的排放,增加了非萜类衍生物。从 EpS/L 获得的主要化合物为()-8-十二烯-1-醇和 1-十五烯,而大根香叶烯 D 和 β-石竹烯则是 EpC 和 EpR 的关键化合物。本工作首次表明,内生细菌会改变重要药用植物感染和未感染植物的香气特征。因此,我们的感染模型可以选择内生菌株,作为生物技术工具,用于生产富含挥发性生物活性化合物的药用植物。

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