Department of Biology, University of Florence, Sesto Fiorentino, Italy.
Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
Nat Prod Res. 2020 Aug;34(15):2232-2237. doi: 10.1080/14786419.2019.1579810. Epub 2019 Mar 25.
The differences in volatile profile of plants not-inoculated (EpC) and inoculated with their endophytes from roots (EpR) and stem/leaves (EpS/L) were analysed and compared by GC-FID/GC-MS in an model. Non-terpenes and sesquiterpene hydrocarbons were the most abundant classes with an opposite behaviour of EpS/L showing a decreased emission of sesquiterpenes and an increase of non-terpene derivatives. The main compounds obtained from EpS/L were ()-8-dodecen-1-ol and 1-pentadecene, while germacrene D and β-caryophyllene were the key compounds in EpC and EpR. For the first time, this work indicates that bacterial endophytes modify the aroma profiles of infected and non-infected plants of the important medicinal plant . Therefore, our model of infection could permit to select endophytic strains to use as biotechnological tool in the production of medicinal plants enriched in volatile bioactive compounds.
采用 GC-FID/GC-MS 对模型中未接种(EpC)和接种内生菌(EpR 和 EpS/L)的根、茎/叶植物的挥发性成分进行分析和比较。非萜类和倍半萜烃是含量最丰富的两类化合物,其中 EpS/L 的表现出相反的行为,即减少了倍半萜的排放,增加了非萜类衍生物。从 EpS/L 获得的主要化合物为()-8-十二烯-1-醇和 1-十五烯,而大根香叶烯 D 和 β-石竹烯则是 EpC 和 EpR 的关键化合物。本工作首次表明,内生细菌会改变重要药用植物感染和未感染植物的香气特征。因此,我们的感染模型可以选择内生菌株,作为生物技术工具,用于生产富含挥发性生物活性化合物的药用植物。