Chiellini Carolina, Maida Isabel, Emiliani Giovanni, Mengoni Alessio, Mocali Stefano, Fabiani Arturo, Biffi Sauro, Maggini Valentina, Gori Luigi, Vannacci Alfredo, Gallo Eugenia, Firenzuoli Fabio, Fani Renato
Department of Biology, University of Florence, Florence, Italy.
Agrobiology and Pedology Research Center, Agricultural Research Council, Florence, Italy.
Int Microbiol. 2014 Sep;17(3):165-74. doi: 10.2436/20.1501.01.219.
In this work we analyzed the composition and structure of cultivable bacterial communities isolated from the stem/leaf and root compartments of two medicinal plants, Echinacea purpurea (L.) Moench and Echinacea angustifolia (DC.) Hell, grown in the same soil, as well as the bacterial community from their rhizospheric soils. Molecular PCR-based techniques were applied to cultivable bacteria isolated from the three compartments of the two plants. The results showed that the two plants and their respective compartments were characterized by different communities, indicating a low degree of strain sharing and a strong selective pressure within plant tissues. Pseudomonas was the most highly represented genus, together with Actinobacteria and Bacillus spp. The presence of distinct bacterial communities in different plant species and among compartments of the same plant species could account for the differences in the medicinal properties of the two plants.
在本研究中，我们分析了从生长于同一土壤中的两种药用植物紫锥菊（Echinacea purpurea (L.) Moench）和狭叶紫锥菊（Echinacea angustifolia (DC.) Hell）的茎/叶和根部分离出的可培养细菌群落的组成和结构，以及它们根际土壤中的细菌群落。基于PCR的分子技术应用于从这两种植物的三个部分分离出的可培养细菌。结果表明，这两种植物及其各自的部分具有不同的群落特征，表明植物组织内菌株共享程度较低且存在强烈的选择压力。假单胞菌属是最具代表性的属，放线菌属和芽孢杆菌属也较为常见。不同植物物种以及同一植物物种不同部分中存在不同的细菌群落，这可能解释了这两种植物药用特性的差异。
