Sanna Giuseppina, Madeddu Silvia, Serra Alessandra, Collu David, Efferth Thomas, Hakkim F Lukmanul, Rashan Luay
Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, Monserrato, Italy.
Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany.
Nat Prod Res. 2021 Feb;35(4):633-636. doi: 10.1080/14786419.2019.1582046. Epub 2019 Mar 25.
(NO), a member of the Apocynaceae family, is an ornamental plant. In this study, we evaluated the antiviral activity of hot and cold extract of NO against six different viruses such as herpes simplex virus type 1 (HSV-1), polio virus type 1 (Sb-1), vesicular stomatitis virus (VSV), reovirus type-1 (Reo-1), human immunodeficiency virus type-1 (HIV-1), and yellow fever virus (YFV). Interestingly the results of plaque reduction assay demonstrated that both, hot extract and cold extract (breastin) of NO inhibited Sb-1 viral infection.In order to identify the mechanism by which NO exerts its antiviral activity, the virucidal effect, the time of addition and the adsorption assay were carried out. Results demonstrated that NO exerts its effect after infection period, particularly during the first two hours post infection.
夹竹桃（夹竹桃科植物）是一种观赏植物。在本研究中，我们评估了夹竹桃的热提取物和冷提取物对六种不同病毒的抗病毒活性，这些病毒包括1型单纯疱疹病毒（HSV - 1）、1型脊髓灰质炎病毒（Sb - 1）、水疱性口炎病毒（VSV）、1型呼肠孤病毒（Reo - 1）、1型人类免疫缺陷病毒（HIV - 1）和黄热病毒（YFV）。有趣的是，蚀斑减少试验结果表明，夹竹桃的热提取物和冷提取物（夹竹桃素）均能抑制Sb - 1病毒感染。为了确定夹竹桃发挥抗病毒活性的机制，我们进行了杀病毒效果、添加时间和吸附试验。结果表明，夹竹桃在感染期后发挥作用，特别是在感染后的头两个小时内。
