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无配体银纳米颗粒：一种对抗病毒和细菌的创新策略。

Ligand-Free Silver Nanoparticles: An Innovative Strategy against Viruses and Bacteria.

作者信息

Morone Maria Vittoria, Chianese Annalisa, Dell'Annunziata Federica, Folliero Veronica, Lamparelli Erwin Pavel, Della Porta Giovanna, Zannella Carla, De Filippis Anna, Franci Gianluigi, Galdiero Massimiliano, Morone Antonio

机构信息

Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania "L. Vanvitelli", 80138 Naples, Italy.

Department of Medicine, Surgery and Dentistry "Scuola Medica Salernitana", 84081 Baronissi, Italy.

出版信息

Microorganisms. 2024 Apr 18;12(4):820. doi: 10.3390/microorganisms12040820.

DOI:10.3390/microorganisms12040820
PMID:38674764
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11052337/
Abstract

The spread of antibiotic-resistant bacteria and the rise of emerging and re-emerging viruses in recent years constitute significant public health problems. Therefore, it is necessary to develop new antimicrobial strategies to overcome these challenges. Herein, we describe an innovative method to synthesize ligand-free silver nanoparticles by Pulsed Laser Ablation in Liquid (PLAL-AgNPs). Thus produced, nanoparticles were characterized by total X-ray fluorescence, zeta potential analysis, transmission electron microscopy (TEM), and nanoparticle tracking analysis (NTA). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to evaluate the nanoparticles' cytotoxicity. Their potential was evaluated against the enveloped herpes simplex virus type 1 (HSV-1) and the naked poliovirus type 1 (PV-1) by plaque reduction assays and confirmed by real-time PCR and fluorescence microscopy, showing that nanoparticles interfered with the early stage of infection. Their action was also examined against different bacteria. We observed that the PLAL-AgNPs exerted a strong effect against both methicillin-resistant ( MRSA) and () producing extended-spectrum β-lactamase (ESBL). In detail, the PLAL-AgNPs exhibited a bacteriostatic action against and a bactericidal activity against . Finally, we proved that the PLAL-AgNPs were able to inhibit/degrade the biofilm of and .

摘要

近年来，抗生素耐药细菌的传播以及新出现和再次出现的病毒的增加构成了重大的公共卫生问题。因此，有必要开发新的抗菌策略来应对这些挑战。在此，我们描述了一种通过液体脉冲激光烧蚀（PLAL-AgNPs）合成无配体银纳米颗粒的创新方法。如此制备的纳米颗粒通过全X射线荧光、zeta电位分析、透射电子显微镜（TEM）和纳米颗粒跟踪分析（NTA）进行表征。进行了3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）试验以评估纳米颗粒的细胞毒性。通过蚀斑减少试验评估了它们对包膜的单纯疱疹病毒1型（HSV-1）和无包膜的脊髓灰质炎病毒1型（PV-1）的潜力，并通过实时PCR和荧光显微镜进行了确认，结果表明纳米颗粒干扰了感染的早期阶段。还检测了它们对不同细菌的作用。我们观察到PLAL-AgNPs对耐甲氧西林金黄色葡萄球菌（MRSA）和产超广谱β-内酰胺酶（ESBL）的细菌均有强烈作用。详细地说，PLAL-AgNPs对[具体细菌1]表现出抑菌作用，对[具体细菌2]表现出杀菌活性。最后，我们证明了PLAL-AgNPs能够抑制/降解[具体细菌1]和[具体细菌2]的生物膜。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/546a9ed3c751/microorganisms-12-00820-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/562d1f701adb/microorganisms-12-00820-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/1b26b4b5d8c7/microorganisms-12-00820-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/0e2da21e5f8b/microorganisms-12-00820-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/2159f9e2e57f/microorganisms-12-00820-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/f353f296d0a2/microorganisms-12-00820-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/2720cd24c507/microorganisms-12-00820-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/f3f73b02a4d2/microorganisms-12-00820-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/33d157ae7217/microorganisms-12-00820-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/b360913e6d37/microorganisms-12-00820-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/3b2983c6606c/microorganisms-12-00820-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/8626c6e022e6/microorganisms-12-00820-g011a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/f52a810791a3/microorganisms-12-00820-g012a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/546a9ed3c751/microorganisms-12-00820-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/562d1f701adb/microorganisms-12-00820-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/1b26b4b5d8c7/microorganisms-12-00820-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/0e2da21e5f8b/microorganisms-12-00820-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/2159f9e2e57f/microorganisms-12-00820-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/f353f296d0a2/microorganisms-12-00820-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/2720cd24c507/microorganisms-12-00820-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/f3f73b02a4d2/microorganisms-12-00820-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/33d157ae7217/microorganisms-12-00820-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/b360913e6d37/microorganisms-12-00820-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/3b2983c6606c/microorganisms-12-00820-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/8626c6e022e6/microorganisms-12-00820-g011a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/f52a810791a3/microorganisms-12-00820-g012a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc7e/11052337/546a9ed3c751/microorganisms-12-00820-g013.jpg

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