School of Environmental Science and Engineering, Shanghai University, Shanghai, China.
Lab for Non-coding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai, China.
Acta Biochim Biophys Sin (Shanghai). 2019 Apr 1;51(4):393-401. doi: 10.1093/abbs/gmz022.
MicroRNAs (miRNAs) are short (20-23 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. In recent years, deep sequencing of the transcription is being increasingly utilized with the promise of higher sensitivity for the identification of differential expression patterns as well as the opportunity to discover new transcripts, including new alternative isoforms and miRNAs. In this study, miRNAs from A549 cells treated with/without rapamycin or starvation were subject to genome-wide deep sequencing. A total of 1534 miRNAs were detected from the rapamycin- and starvation-treated A549 cells. Among them, 31 miRNAs were consistently upregulated and 131 miRNAs were downregulated in the treated cells when compared with the untreated cells. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis of the predicted target genes of the most significantly differentially expressed miRNAs revealed that the autophagy-related miRNAs are involved in cancer pathway. Taken together, our findings indicate that the underlying mechanism responsible for autophagy is associated with dysregulation of miRNAs in rapamycin- or starvation-induced A549 cells.
MicroRNAs (miRNAs) 是短链 (20-23nt) 的非编码 RNA，通过影响 mRNA 的稳定性和翻译，参与多细胞生物中转录后基因表达的调控。近年来，随着转录组深度测序技术的日益普及，人们越来越希望通过该技术提高差异表达模式识别的灵敏度，以及发现新的转录本，包括新的选择性异构体和 miRNAs。在这项研究中，对用雷帕霉素或饥饿处理的 A549 细胞中的 miRNAs 进行了全基因组深度测序。从雷帕霉素和饥饿处理的 A549 细胞中总共检测到 1534 个 miRNAs。其中，与未处理细胞相比，处理细胞中有 31 个 miRNAs 持续上调，131 个 miRNAs 下调。对差异表达最显著的 miRNAs 的预测靶基因进行基因本体论和京都基因与基因组百科全书通路分析表明，与自噬相关的 miRNAs 参与了癌症通路。总之，我们的研究结果表明，雷帕霉素或饥饿诱导的 A549 细胞中自噬的潜在机制与 miRNAs 的失调有关。
