通过氨基功能化硅量子点实现无酶葡萄糖传感。

Enzyme free glucose sensing by amino-functionalized silicon quantum dot.

机构信息

Institute of Environmental Science, School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jun 5;216:303-309. doi: 10.1016/j.saa.2019.03.071. Epub 2019 Mar 19.

DOI:10.1016/j.saa.2019.03.071

PMID:30909086

Abstract

Silicon quantum dots have become one of the most popular nanomaterials in biological applications for their excellent biocompatibility and optical properties. Herein, we synthesized amino-functionalized silicon quantum dots (NH@SiQDs) via a simple microemulsion method, in which silicon tetrachloride and allylamine were used as source of silicon and functional group. NH@SiQDs exhibits good water-solubility, high fluorescence quantum yield and optical stability. A non-enzymatic biosensor of glucose was developed based on the fluorescence quenching of NH@SiQDs in response to glucose. The fluorescence response was linearly proportional to glucose in the concentration range of 1.0 × 10-9.0 × 10 mol/L and the detection limit was determined to be 3.0 × 10 mol/L. The developed glucose sensor was successfully applied in blood glucose analysis of human serum. Satisfactory result that agreed very well with traditional method was obtained.

摘要

硅量子点因其优异的生物相容性和光学性质，已成为生物应用中最受欢迎的纳米材料之一。在此，我们通过简单的微乳液法合成了氨基功能化硅量子点（NH@SiQDs），其中四氯化硅和丙烯胺分别作为硅源和功能基团。NH@SiQDs 具有良好的水溶性、高荧光量子产率和光学稳定性。基于 NH@SiQDs 对葡萄糖的荧光猝灭响应，构建了一种非酶葡萄糖生物传感器。该传感器在 1.0×10-9.0×10-6 mol/L 浓度范围内对葡萄糖的荧光响应呈线性关系，检测限为 3.0×10-6 mol/L。该葡萄糖传感器已成功应用于人血清中血糖的分析，获得了与传统方法非常吻合的令人满意的结果。