长链非编码 RNA PVT1 在脑胶质瘤中的表达的临床意义。

Clinical significance of the expression of long non-coding RNA PVT1 in glioma.

出版信息

Cancer Biomark. 2019;24(4):509-513. doi: 10.3233/CBM-182253.

DOI:10.3233/CBM-182253

PMID:30909189

Abstract

BACKGROUND

Glioma is the most common primary malignant tumor in the nervous system.

OBJECTIVE

To investigate the expression of long non-coding RNA Pvt1 oncogene (PVT1) in glioma and its clinical significance.

METHODS

The expression levels of PVT1 were determined in 59 glioma and 10 normal tissue samples using qRT-PCR. The patients were divided into high and low expression groups and analyzed for their relationship with clinicopathological factors and the survival time using the Kaplan-Meier method.

RESULTS

The expression levels of PVT1 were significantly higher in glioma tissues than in normal tissues (p< 0.01) and higher in high grade (III-IV) than in low grade (I-II) tumors (p< 0.01). Analysis showed that the PVT1 level was closely related to glioma grade (p< 0.01), but not to age, gender, Karnofsky performance status (KPS) and tumor size (p> 0.05). Receiver operator characteristic curve analysis showed an area under the curve of 0.835. Log-rank test showed that the prognosis of high PVT1 group was poorer than that of low PVT1 group (p< 0.01).

CONCLUSIONS

PVT1 is highly expressed in gliomas and its level is positively related to WHO glioma grade and prognosis of gliomas. Therefore, it may be explored as a new molecular marker for predicting malignancy and prognosis of gliomas.

摘要

背景

脑胶质瘤是神经系统最常见的原发性恶性肿瘤。

目的

探讨长链非编码 RNA Pvt1 癌基因（PVT1）在脑胶质瘤中的表达及其临床意义。

方法

采用 qRT-PCR 检测 59 例脑胶质瘤组织和 10 例正常脑组织中 PVT1 的表达水平。根据 PVT1 的表达水平将患者分为高表达组和低表达组，采用 Kaplan-Meier 法分析其与临床病理因素及生存时间的关系。

结果

PVT1 在脑胶质瘤组织中的表达水平明显高于正常脑组织（p<0.01），且在高级别（III-IV 级）肿瘤中高于低级别（I-II 级）肿瘤（p<0.01）。分析表明，PVT1 水平与脑胶质瘤分级密切相关（p<0.01），但与年龄、性别、卡氏功能状态评分（KPS）和肿瘤大小无关（p>0.05）。受试者工作特征曲线分析显示曲线下面积为 0.835。Log-rank 检验显示，PVT1 高表达组的预后较 PVT1 低表达组差（p<0.01）。