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一种使用固相葡萄球菌蛋白的快速载脂蛋白E放射免疫测定法。使用混合血浆作为二级标准品。

A rapid apolipoprotein E radioimmunoassay using solid-phase staphylococcus protein. Use of pooled plasma as a secondary standard.

作者信息

Au Y P, Bren N D, Kottke B A

出版信息

Biochem Biophys Res Commun. 1986 Jul 16;138(1):455-62. doi: 10.1016/0006-291x(86)90302-5.

DOI:10.1016/0006-291x(86)90302-5
PMID:3091017
Abstract

A rapid apolipoprotein E (apo E) radioimmunoassay, which requires a total of 24 hour incubation as compared to the usual 3-5 days, has been developed in our laboratory. Solid phase staphylococcus protein A was used to separate bound and unbound labeled antigen. Use of a pooled plasma (quality control sample) as a secondary standard to reduce interassay variation was also described.

摘要

我们实验室已开发出一种快速载脂蛋白E(apo E)放射免疫测定法,与通常的3 - 5天相比,该方法总共只需24小时孵育。使用固相葡萄球菌蛋白A分离结合和未结合的标记抗原。还介绍了使用混合血浆(质量控制样品)作为二级标准以减少测定间差异的方法。

相似文献

1
A rapid apolipoprotein E radioimmunoassay using solid-phase staphylococcus protein. Use of pooled plasma as a secondary standard.一种使用固相葡萄球菌蛋白的快速载脂蛋白E放射免疫测定法。使用混合血浆作为二级标准品。
Biochem Biophys Res Commun. 1986 Jul 16;138(1):455-62. doi: 10.1016/0006-291x(86)90302-5.
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Use of a quality-control plasma sample to decrease interassay variation in radioimmunoassays of apolipoprotein A-I.
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Radioimmunoassay of apolipoprotein A-i. application of a non-ionic detergent (Tween-20) and solid-phase staphylococcus.载脂蛋白A - I的放射免疫测定。非离子去污剂(吐温 - 20)和固相葡萄球菌的应用。
Clin Chem. 1982 Jan;28(1):199-204.
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[Plasma apolipoproteins: their role in cardiovascular diseases and usefulness of their blood concentration determination. Committee of the Study of Apolipoproteins, Italian Heart Foundation].[血浆载脂蛋白:它们在心血管疾病中的作用及其血液浓度测定的用途。意大利心脏基金会载脂蛋白研究委员会]
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Lipids Health Dis. 2013 Mar 27;12:43. doi: 10.1186/1476-511X-12-43.
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Effect of 25-hydroxycholesterol and bile acids on the regulation of cholesterol metabolism in Hep G2 cells.25-羟基胆固醇和胆汁酸对Hep G2细胞胆固醇代谢调节的影响
Biochem J. 1989 Nov 15;264(1):241-7. doi: 10.1042/bj2640241.
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Genetic and environmental explanations for the distribution of sodium-lithium countertransport in pedigrees from Rochester, MN.
明尼苏达州罗切斯特市家系中钠-锂逆向转运分布的遗传和环境解释。
Am J Hum Genet. 1991 Jun;48(6):1092-104.
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Baculovirus-mediated expression of human apolipoprotein E in Manduca sexta larvae generates particles that bind to the low density lipoprotein receptor.杆状病毒介导的人载脂蛋白E在烟草天蛾幼虫中的表达产生了与低密度脂蛋白受体结合的颗粒。
Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8530-3. doi: 10.1073/pnas.88.19.8530.
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The gender-specific apolipoprotein E genotype influence on the distribution of lipids and apolipoproteins in the population of Rochester, MN. I. Pleiotropic effects on means and variances.性别特异性载脂蛋白E基因型对明尼苏达州罗切斯特市人群中脂质和载脂蛋白分布的影响。I. 对均值和方差的多效性作用。
Am J Hum Genet. 1991 Dec;49(6):1155-66.