Au Y P, Bren N D, Kottke B A
Biochem Biophys Res Commun. 1986 Jul 16;138(1):455-62. doi: 10.1016/0006-291x(86)90302-5.
A rapid apolipoprotein E (apo E) radioimmunoassay, which requires a total of 24 hour incubation as compared to the usual 3-5 days, has been developed in our laboratory. Solid phase staphylococcus protein A was used to separate bound and unbound labeled antigen. Use of a pooled plasma (quality control sample) as a secondary standard to reduce interassay variation was also described.
我们实验室已开发出一种快速载脂蛋白E(apo E)放射免疫测定法,与通常的3 - 5天相比,该方法总共只需24小时孵育。使用固相葡萄球菌蛋白A分离结合和未结合的标记抗原。还介绍了使用混合血浆(质量控制样品)作为二级标准以减少测定间差异的方法。