Department of General Surgical Science, Gunma University, Graduate School of Medicine, Gunma, Japan.
Department of Pathology, Gunma University Hospital, Gunma, Japan.
Ann Thorac Surg. 2019 Jul;108(1):235-243. doi: 10.1016/j.athoracsur.2019.02.040. Epub 2019 Mar 22.
Stathmin-1 regulates microtubule dynamics and is associated with malignant phenotypes in non-small cell lung cancer (NSCLC). This study evaluated its diagnostic value for differentiating between NSCLC and high-grade lung neuroendocrine tumor (HGNET).
Stathmin-1 protein expression was assessed by immunohistochemistry in 414 NSCLC (305 adenocarcinoma [AD], 102 squamous cell carcinoma [SCC], 7 large-cell carcinoma), 5 typical carcinoid (low-grade lung neuroendocrine tumor), and 34 HGNET (17 small-cell carcinoma [SCLC] and 17 large-cell neuroendocrine carcinoma [LCNEC]) surgical specimens and 57 NSCLC (29 AD and 28 SCC) and 42 HGNET (17 LCNEC and 25 SCLC) biopsy specimens. We also analyzed stathmin-1 mRNA levels in 81 NSCLCs and 26 HGNETs with the use of reverse transcription-polymerase chain reaction.
Among NSCLC samples, we saw high stathmin-1 protein expression in only three ADs, one SCC, and one large-cell carcinoma surgical samples, all five of which showed neuroendocrine characteristics in pathologic re-review; and low or intermediate expression in all five typical carcinoid surgical samples and all 57 NSCLC biopsy samples. In contrast, all HGNET surgical (n = 34) and biopsy (n = 42) samples showed high stathmin-1 expression. In reverse transcription-polymerase chain reaction, stathmin-1 expression was significantly higher in HGNET tissues than in NSCLC tissues (p < 0.001).
Stathmin-1 expression can help in differentiating NSCLC from HGNET.
Stathmin-1 调节微管动力学，与非小细胞肺癌（NSCLC）中的恶性表型相关。本研究评估了其用于鉴别 NSCLC 和高级别肺神经内分泌肿瘤（HGNET）的诊断价值。
采用免疫组织化学法检测 414 例 NSCLC（305 例腺癌[AD]，102 例鳞状细胞癌[SCC]，7 例大细胞癌）、5 例典型类癌（低级别肺神经内分泌肿瘤）和 34 例 HGNET（17 例小细胞癌[SCLC]和 17 例大细胞神经内分泌癌[LCNEC]）手术标本以及 57 例 NSCLC（29 例 AD 和 28 例 SCC）和 42 例 HGNET（17 例 LCNEC 和 25 例 SCLC）活检标本中的 Stathmin-1 蛋白表达。我们还使用逆转录-聚合酶链反应分析了 81 例 NSCLCs 和 26 例 HGNETs 的 Stathmin-1 mRNA 水平。
在 NSCLC 样本中，我们仅在 3 例 AD、1 例 SCC 和 1 例大细胞癌手术样本中观察到高 Stathmin-1 蛋白表达，这 5 例在病理复查中均显示出神经内分泌特征；而在所有 5 例典型类癌手术样本和所有 57 例 NSCLC 活检样本中均表现为低或中等表达。相比之下，所有 HGNET 手术（n=34）和活检（n=42）样本均显示出高 Stathmin-1 表达。在逆转录-聚合酶链反应中，HGNET 组织中的 Stathmin-1 表达明显高于 NSCLC 组织（p<0.001）。
Stathmin-1 表达有助于鉴别 NSCLC 和 HGNET。
