Stathmin-1 是一种用于诊断高级别肺神经内分泌肿瘤的有用标志物。

Stathmin-1 Is a Useful Diagnostic Marker for High-Grade Lung Neuroendocrine Tumors.

机构信息

Department of General Surgical Science, Gunma University, Graduate School of Medicine, Gunma, Japan.

Department of Pathology, Gunma University Hospital, Gunma, Japan.

出版信息

Ann Thorac Surg. 2019 Jul;108(1):235-243. doi: 10.1016/j.athoracsur.2019.02.040. Epub 2019 Mar 22.

DOI:10.1016/j.athoracsur.2019.02.040

PMID:30910656

Abstract

BACKGROUND

Stathmin-1 regulates microtubule dynamics and is associated with malignant phenotypes in non-small cell lung cancer (NSCLC). This study evaluated its diagnostic value for differentiating between NSCLC and high-grade lung neuroendocrine tumor (HGNET).

METHODS

Stathmin-1 protein expression was assessed by immunohistochemistry in 414 NSCLC (305 adenocarcinoma [AD], 102 squamous cell carcinoma [SCC], 7 large-cell carcinoma), 5 typical carcinoid (low-grade lung neuroendocrine tumor), and 34 HGNET (17 small-cell carcinoma [SCLC] and 17 large-cell neuroendocrine carcinoma [LCNEC]) surgical specimens and 57 NSCLC (29 AD and 28 SCC) and 42 HGNET (17 LCNEC and 25 SCLC) biopsy specimens. We also analyzed stathmin-1 mRNA levels in 81 NSCLCs and 26 HGNETs with the use of reverse transcription-polymerase chain reaction.

RESULTS

Among NSCLC samples, we saw high stathmin-1 protein expression in only three ADs, one SCC, and one large-cell carcinoma surgical samples, all five of which showed neuroendocrine characteristics in pathologic re-review; and low or intermediate expression in all five typical carcinoid surgical samples and all 57 NSCLC biopsy samples. In contrast, all HGNET surgical (n = 34) and biopsy (n = 42) samples showed high stathmin-1 expression. In reverse transcription-polymerase chain reaction, stathmin-1 expression was significantly higher in HGNET tissues than in NSCLC tissues (p < 0.001).

CONCLUSIONS

Stathmin-1 expression can help in differentiating NSCLC from HGNET.

摘要

背景

Stathmin-1 调节微管动力学，与非小细胞肺癌（NSCLC）中的恶性表型相关。本研究评估了其用于鉴别 NSCLC 和高级别肺神经内分泌肿瘤（HGNET）的诊断价值。

方法

采用免疫组织化学法检测 414 例 NSCLC（305 例腺癌[AD]，102 例鳞状细胞癌[SCC]，7 例大细胞癌）、5 例典型类癌（低级别肺神经内分泌肿瘤）和 34 例 HGNET（17 例小细胞癌[SCLC]和 17 例大细胞神经内分泌癌[LCNEC]）手术标本以及 57 例 NSCLC（29 例 AD 和 28 例 SCC）和 42 例 HGNET（17 例 LCNEC 和 25 例 SCLC）活检标本中的 Stathmin-1 蛋白表达。我们还使用逆转录-聚合酶链反应分析了 81 例 NSCLCs 和 26 例 HGNETs 的 Stathmin-1 mRNA 水平。

结果

在 NSCLC 样本中，我们仅在 3 例 AD、1 例 SCC 和 1 例大细胞癌手术样本中观察到高 Stathmin-1 蛋白表达，这 5 例在病理复查中均显示出神经内分泌特征；而在所有 5 例典型类癌手术样本和所有 57 例 NSCLC 活检样本中均表现为低或中等表达。相比之下，所有 HGNET 手术（n=34）和活检（n=42）样本均显示出高 Stathmin-1 表达。在逆转录-聚合酶链反应中，HGNET 组织中的 Stathmin-1 表达明显高于 NSCLC 组织（p<0.001）。