Rong Biaoxue, Nan Yandong, Liu Hua, Gao Wenlong
Department of Respiratory Medicine, First Affiliated Hospital, Xi'an Medical University, Xi'an 710077, Shaanxi, China.
Department of Respiratory Medicine, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
Cancer Biomark. 2017;19(1):35-43. doi: 10.3233/CBM-160239.
Previous studies show that overexpression of stathmin involved in the malignant biological behavior of lung cancer. This investigation is to disclose the expression status of stathmin in non-small cell lung cancer (NSCLC) and its clinical value for the diagnosis and prognosis to lung cancer.
The expression of stathmin in cells and tissues of NSCLC was examined using immunohistochemistry (IHC), in-situ hybridization (ISH), and Western blot. The correlation between stathmin expression and survival of lung cancer patients was evaluated by a Kaplan-Meier method and the multiple regression analysis.
NSCLC tissues and cells showed an overexpression of stathmin compared with normal lung tissues and cells (p< 0.05). And the expression level of stathmin was significantly associated with lung adenocarcinoma (LAC) (p< 0.05), lymphatic invasion (p< 0.05) and advanced stages of NSCLC (p< 0.05). Moreover, overexpression of stathmin predicted a reduced survival (p<0.05).
Increased stathmin correlated with pathologic grade, lymphatic invasion, advanced stage and poor survival of NSCLC, which indicated that stathmin could serve as a potential biomarker of NSCLC.
既往研究表明，Stathmin的过表达与肺癌的恶性生物学行为有关。本研究旨在揭示Stathmin在非小细胞肺癌（NSCLC）中的表达状况及其对肺癌诊断和预后的临床价值。
采用免疫组织化学（IHC）、原位杂交（ISH）和蛋白质免疫印迹法检测NSCLC细胞和组织中Stathmin的表达。采用Kaplan-Meier法和多元回归分析评估Stathmin表达与肺癌患者生存率之间的相关性。
与正常肺组织和细胞相比，NSCLC组织和细胞中Stathmin呈过表达（p < 0.05）。且Stathmin的表达水平与肺腺癌（LAC）（p < 0.05）、淋巴浸润（p < 0.05）及NSCLC晚期（p < 0.05）显著相关。此外，Stathmin的过表达预示着生存率降低（p < 0.05）。
Stathmin表达增加与NSCLC的病理分级、淋巴浸润、晚期及不良生存相关，这表明Stathmin可作为NSCLC的潜在生物标志物。
