Department of Neurology, Affiliated Hospital of Zunyi Medical University, No. 149 Dalian Road, Zunyi, Guizhou, China, 563003.
Key Laboratory of Basic Pharmacology of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou, China, 563003.
Int J Med Sci. 2019 Jan 29;16(3):394-402. doi: 10.7150/ijms.28359. eCollection 2019.
Synaptophysin (SYP) and growth-associated binding protein 43 (GAP-43) have been shown to be closely related to hippocampal synaptic plasticity in recent years. They are important molecular markers associated with synaptic plasticity. However, the role of SYP and GAP-43 in chronic intermittent hypoxic injury of the central nervous system needs to be further clarified. In this study, 25 adult male sprague dawley (SD) rats were randomly divided into a normal control group (CON) and a chronic intermittent hypoxia group (CIH) with four time points as follows: 1 W, 2 W, 3 W, and 4 W. The behavioural changes (primarily learning and memory abilities) were observed by the Morris water maze in each group, consisting of 5 rats per group.The localization of SYP and GAP-43 in hippocampal CA1 neurons was observed, and the expression of SYP and GAP-43 in the hippocampus was detected by Western blotting. The results showed that the mean oxygen saturation of the tail artery in CIH rats was less than that in normal rats (P < 0.05). The escape latency of CIH rats was longer than that of normal rats, and the number of space exploration platform crossings was less than that of normal rats. SYP-positive stained cells were yellow or brown and were mainly expressed on the cell membrane, while the GAP-43-positive staining was brown and was mainly expressed on the cell membrane and in the cytoplasm. The expression of SYP in plasma decreased gradually at the four time points for the CIH group (P < 0.05), while the expression of GAP-43 in the CIH 1W group increased (P < 0.05) and decreased gradually in the CIH 2 W, CIH 3 W and CIH 4 W groups (P < 0.05).
近年来，突触素（SYP）和生长相关结合蛋白 43（GAP-43）被证明与海马突触可塑性密切相关。它们是与突触可塑性相关的重要分子标志物。然而，SYP 和 GAP-43 在中枢神经系统慢性间歇性缺氧损伤中的作用尚需进一步阐明。在这项研究中，将 25 只成年雄性 Sprague Dawley（SD）大鼠随机分为正常对照组（CON）和慢性间歇性低氧组（CIH），每组四个时间点：1W、2W、3W 和 4W。每组 5 只大鼠通过 Morris 水迷宫观察行为变化（主要是学习和记忆能力）。观察海马 CA1 神经元中 SYP 和 GAP-43 的定位，并用 Western blot 检测海马中 SYP 和 GAP-43 的表达。结果显示，CIH 大鼠尾动脉平均氧饱和度低于正常大鼠（P<0.05）。CIH 大鼠的逃避潜伏期长于正常大鼠，空间探索平台穿越次数少于正常大鼠。SYP 阳性染色细胞呈黄色或棕色，主要表达在细胞膜上，而 GAP-43 阳性染色呈棕色，主要表达在细胞膜和细胞质上。CIH 组四个时间点血浆中 SYP 的表达逐渐降低（P<0.05），而 CIH 1W 组 GAP-43 的表达增加（P<0.05），CIH 2W、CIH 3W 和 CIH 4W 组的表达逐渐降低（P<0.05）。
