Papiernik M, Jacobson J B
Cell Immunol. 1986 Jan;97(1):23-33. doi: 10.1016/0008-8749(86)90371-0.
In the present paper, the response of thymocytes to Con A is analyzed in terms of a cooperative phenomenon between medullary thymocytes, cortical thymocytes, thymic accessory cells, and interleukin 2. Medullary thymocytes respond spontaneously to Con A and produce IL-2. The addition of exogenously produced IL-2 enhances their proliferation. Small numbers of cortical (PNA+) thymocytes do not respond to Con A, even in the presence of IL-2-containing supernatant. By increasing the number of PNA+ cells per well, sensitivity to Con A and IL-2 appears. This response may be linked either to the increase in a minor PNA+-responding population and/or to the enhanced contamination by medullary thymocytes and macrophages in non-responding PNA+ thymocyte population. In this hypothesis, either the contaminating cells respond by themselves and/or cooperate with PNA+ cells to induce their proliferation. Coculture of non-responding low numbers of PNA+ thymocytes with Con A- and IL-2-containing supernatant in the presence of PNA- cells containing thymic medullary thymocytes and macrophages always produces a higher response than that of each individual population. These results show that a cooperative phenomenon occurs in the cocultures of PNA+ and PNA- thymic cells. We can show using PNA+ and PNA- thymocytes with different Thy 1 alleles, that indeed both PNA+ and populations participate PNA-thymocytes with different Thy 1 alleles, that indeed both PNA+ and PNA- populations participate in the generation of proliferating cells. We can demonstrate, by lysis experiments with monoclonal antibodies and complement that at the end of coculture, most of the proliferating cells are Lyt 1+, and part are Lyt 2+ or L3T4+. We discuss the fact that the phenotype of the cells after activation does not allow us to deduce the phenotype of their precursors. Lysis of Ia+ cells prior to coculture, reduces the level of the proliferative response but does not modify the percentage of cooperation produced by the coculture. Cooperation with medullary mature thymocytes or the presence of active Ia- accessory cells possibly able to convert to Ia expression during coculture experiments may account for these results.
在本论文中，依据髓质胸腺细胞、皮质胸腺细胞、胸腺辅助细胞和白细胞介素2之间的协同现象，分析了胸腺细胞对刀豆蛋白A的反应。髓质胸腺细胞对刀豆蛋白A有自发反应并产生白细胞介素2。添加外源性产生的白细胞介素2可增强其增殖。少量皮质（PNA +）胸腺细胞即使在含有白细胞介素2的上清液存在下也对刀豆蛋白A无反应。通过增加每孔中PNA +细胞的数量，对刀豆蛋白A和白细胞介素2的敏感性出现。这种反应可能与少数PNA +反应群体的增加和/或与无反应的PNA +胸腺细胞群体中髓质胸腺细胞和巨噬细胞污染的增加有关。在这个假设中，要么污染细胞自身反应和/或与PNA +细胞合作诱导其增殖。在含有胸腺髓质胸腺细胞和巨噬细胞的PNA -细胞存在下，将少量无反应的PNA +胸腺细胞与含有刀豆蛋白A和白细胞介素2的上清液共培养，总是产生比每个单独群体更高的反应。这些结果表明，在PNA +和PNA -胸腺细胞的共培养中发生了协同现象。我们可以使用具有不同Thy 1等位基因的PNA +和PNA -胸腺细胞表明，实际上PNA +和PNA -群体都参与了增殖细胞的产生。我们可以通过用单克隆抗体和补体进行裂解实验证明，在共培养结束时，大多数增殖细胞是Lyt 1 +，部分是Lyt 2 +或L3T4 +。我们讨论了激活后细胞的表型不允许我们推断其前体表型的事实。在共培养之前裂解Ia +细胞会降低增殖反应水平，但不会改变共培养产生的合作百分比。与髓质成熟胸腺细胞的合作或可能在共培养实验期间能够转化为Ia表达的活性Ia -辅助细胞的存在可能解释了这些结果。
