Paire J, Monestier M, Rigal D, Martel F, Desgranges C
Immunol Lett. 1986 Sep;13(3):137-41. doi: 10.1016/0165-2478(86)90046-5.
Two cell lines producing monoclonal antibodies have been established from peripheral blood of a negative Rhesus blood donor which has been immunized with positive Rhesus red blood cells. Two monoclonal antibodies Co II 8.8 and Co II 7.12 have been selected. Both are IgG1 antibodies, but recognize different epitopes on the Rhesus D antigen, apparently associated with different subunits of the D antigen. Thus the Co II 8.8, like the positive serum, immunoprecipitates an antigen of a relative molecular weight of 33 kDa, while the Co II 7.12 recognizes an antigen of Mr 42 kDa.
已从一名阴性恒河猴献血者的外周血中建立了两个产生单克隆抗体的细胞系，该献血者已用阳性恒河猴红细胞进行免疫。已筛选出两种单克隆抗体Co II 8.8和Co II 7.12。两者均为IgG1抗体，但识别恒河猴D抗原上不同的表位，显然与D抗原的不同亚基相关。因此，Co II 8.8与阳性血清一样，能免疫沉淀相对分子质量为33 kDa的抗原，而Co II 7.12识别的抗原的相对分子质量为42 kDa。
