Department of Gastroenterology, The No.1 People's Hospital of Jingmen, Jingmen, China.
Eur Rev Med Pharmacol Sci. 2019 Mar;23(5):2013-2019. doi: 10.26355/eurrev_201903_17240.
Increasing studies have indicated the important functions of long non-coding RNAs (lncRNAs) in tumorigenesis and progression including hepatocellular carcinoma (HCC). The study aims to explore the role of long non-coding RNA AK001796 in HCC progression.
Quantitative Real-time polymerase chain reaction (QRT-PCR) analysis was performed to examine the lncRNA AK001796 expression in 73 cases of human HCC tissue samples and matched adjacent normal tissues. Besides, the relationship between lncRNA AK001796 expression and clinicopathologic characteristics was analyzed. Overall survival (OS) curves of patients were constructed by the Kaplan-Meier methods. Multivariate Cox regression analysis was used to evaluate independent risk factors affecting HCC prognosis. Cell proliferation and invasion abilities are analyzed by cell counting kit-8 (CCK-8) and transwell invasion assays.
We showed that the lncRNA AK001796 expression was significantly up-regulated in HCC tissues and cell lines, compared to their controls, respectively. Higher lncRNA AK001796 expression closely correlated with tumor size (p<0.05), TNM stage (p<0.05) and the poor overall survival (OS) rate of HCC patients (p<0.05). Besides, multivariate Cox regression analysis found that lncRNA AK001796 expression was identified as an independent risk factor for HCC prognosis. In vitro, we showed that lncRNA AK001796 knockdown markedly suppressed cell proliferation and cell invasion abilities.
Our results suggested that lncRNA AK001796 acts as a predictor of HCC prognosis and may provide an important clinical value for HCC treatment.
越来越多的研究表明长链非编码 RNA（lncRNA）在肿瘤发生和发展中具有重要作用，包括肝细胞癌（HCC）。本研究旨在探讨长链非编码 RNA AK001796 在 HCC 进展中的作用。
采用实时定量聚合酶链反应（QRT-PCR）分析 73 例人 HCC 组织样本和配对的相邻正常组织中 lncRNA AK001796 的表达。此外，分析了 lncRNA AK001796 表达与临床病理特征的关系。Kaplan-Meier 方法构建患者总生存（OS）曲线。多变量 Cox 回归分析用于评估影响 HCC 预后的独立危险因素。通过细胞计数试剂盒-8（CCK-8）和 Transwell 侵袭实验分析细胞增殖和侵袭能力。
我们发现，与对照相比，lncRNA AK001796 在 HCC 组织和细胞系中的表达明显上调。较高的 lncRNA AK001796 表达与肿瘤大小（p<0.05）、TNM 分期（p<0.05）和 HCC 患者总体生存率（OS）较差密切相关（p<0.05）。此外，多变量 Cox 回归分析发现，lncRNA AK001796 表达是 HCC 预后的独立危险因素。体外实验表明，lncRNA AK001796 下调显著抑制细胞增殖和细胞侵袭能力。
我们的研究结果表明，lncRNA AK001796 可作为 HCC 预后的预测因子，并可能为 HCC 治疗提供重要的临床价值。
