Department of Hepatobiliary Surgery, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, China (mainland).
Imaging Center, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, China (mainland).
Med Sci Monit. 2019 Aug 31;25:6539-6546. doi: 10.12659/MSM.916551.
BACKGROUND Long non-coding RNAs (lncRNAs) have been shown to play an important regulatory role in many tumors. This study was designed to investigate the expression of lncRNA ENST00000429227.1 in hepatocellular carcinoma (HCC) and to determine whether the expression of lncRNA ENST00000429227.1 affects the prognosis of HCC. MATERIAL AND METHODS lncRNA ENST00000429227.1 showing differences in expression between M1 and M2 was screened by microarray expression measurements. Quantitative real-time PCR (qRT-PCR) was used to detect the expression of lncRNA ENST00000429227.1 in 161 HCC patients. The chi-square test was used to evaluate the relationship between the expression of ENST00000429227.1 and clinicopathological parameters. A survival curve was drawn and analyzed by Kaplan-Meier method. Cox regression was used for univariate and multivariate analysis to determine whether lncRNA ENST00000429227.1 is an independent factor of the occurrence and prognosis of HCC. RESULTS A total of 3703 differentially expressed lncRNAs were obtained, of which 1777 were upregulated and 1926 were downregulated, with multiple change >1.5. The expression of lncRNA ENST00000429227.1 was upregulated in M2 cells. The expression of lncRNA ENST00000429227.1 in HCC tissues was higher than that in adjacent normal tissues (p<0.05), which was correlated with pathological parameters such as surgical margin (p=0.042), AFP (p=0.022) and Barcelona Clinic Liver Cancer (BCLC) stage (p=0.008). Survival analysis showed that high expression of lncRNA ENST00000429227.1 was associated with a decrease in overall survival (OS) rate of HCC patients. Cox regression analysis showed that high expression of ENST00000429227.1 may be an independent risk factor affecting the prognosis of HCC patients. CONCLUSIONS The results suggest that upregulation of ENST00000429227.1 is associated with poor prognosis of HCC patients, and may be a new biomarker for the diagnosis of HCC.
长链非编码 RNA（lncRNA）在许多肿瘤中发挥着重要的调控作用。本研究旨在探讨 lncRNAENST00000429227.1 在肝细胞癌（HCC）中的表达情况，并确定 lncRNAENST00000429227.1 的表达是否影响 HCC 的预后。
通过微阵列表达测量筛选出在 M1 和 M2 之间表达差异的 lncRNAENST00000429227.1。采用实时荧光定量 PCR（qRT-PCR）检测 161 例 HCC 患者中 lncRNAENST00000429227.1 的表达。采用卡方检验评估 ENST00000429227.1 的表达与临床病理参数之间的关系。通过 Kaplan-Meier 法绘制生存曲线并进行分析。采用 Cox 回归进行单因素和多因素分析，以确定 lncRNAENST00000429227.1 是否是 HCC 发生和预后的独立因素。
共获得 3703 个差异表达的 lncRNA，其中 1777 个上调，1926 个下调，变化倍数>1.5。lncRNAENST00000429227.1 在 M2 细胞中表达上调。HCC 组织中 lncRNAENST00000429227.1 的表达高于相邻正常组织（p<0.05），与手术切缘（p=0.042）、甲胎蛋白（p=0.022）和巴塞罗那临床肝癌（BCLC）分期（p=0.008）等病理参数相关。生存分析表明，lncRNAENST00000429227.1 高表达与 HCC 患者总生存率（OS）降低相关。Cox 回归分析表明，ENST00000429227.1 高表达可能是影响 HCC 患者预后的独立危险因素。
研究结果表明，ENST00000429227.1 的上调与 HCC 患者预后不良相关，可能成为 HCC 诊断的新生物标志物。
