From the Department of Pathology, Walter Reed National Military Medical Center, Bethesda Maryland (Dr Borch); the Department of Hematopathology, University of Virginia, Charlottesville (Dr Aguilera); the Department of Hematopathology, Joint Pathology Center, Silver Spring, Maryland (Drs Brissette and Auerbach); and the Department of Hematopathology, NeoGenomics, Aliso Viejo, California (Dr O'Malley).
Arch Pathol Lab Med. 2019 Sep;143(9):1093-1105. doi: 10.5858/arpa.2018-0211-CP. Epub 2019 Mar 27.
CONTEXT.—: Even though immunohistochemistry is routinely used by pathologists, evaluation of immunohistochemistry in splenic lesions remains difficult for many. Classification of benign and splenic lesions often requires a combination of hematoxylin-eosin evaluation, immunophenotyping, and sometimes molecular testing. Immunohistochemical staining is essential in evaluating many splenic lesions, and requires an understanding of the normal compartments of the spleen.
OBJECTIVE.—: To address different immunohistochemical features used for identification and subclassification of different lesions of the spleen, as well as in the normal compartments of the spleen.
DATA SOURCES.—: The information outlined in this review article is based on our experiences with a variety of spleen cases, on the current World Health Organization classification of hematopoietic and lymphoid tumors, and on a review of English-language articles published during 2018.
CONCLUSIONS.—: Features for phenotyping normal spleen as well as a variety of splenic lesions, including littoral cell angioma and splenic marginal zone lymphoma, are discussed. Suggested immunopanels are provided to assist in the diagnosis of different lesions of the spleen.
尽管免疫组织化学已被病理学家广泛应用，但对于许多人来说，评估脾脏病变中的免疫组织化学仍然具有一定难度。为了对良性和脾脏病变进行分类，通常需要综合评估苏木精-伊红染色、免疫表型，有时还需要进行分子检测。免疫组织化学染色在评估许多脾脏病变中至关重要，且需要了解脾脏的正常结构。
探讨用于鉴定和细分不同脾脏病变的不同免疫组织化学特征，以及用于脾脏正常结构的鉴定。
本综述文章中概述的信息基于我们对各种脾脏病例的经验、当前世界卫生组织（WHO）关于造血和淋巴组织肿瘤的分类，以及对 2018 年期间发表的英文文章的回顾。
本文讨论了用于鉴定正常脾脏以及各种脾脏病变（包括边缘区淋巴细胞瘤和血管外皮细胞瘤）的表型的特征。此外，还提供了建议的免疫组化面板，以协助诊断不同的脾脏病变。
