School of Mathematical and Physical Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia.
J Phys Condens Matter. 2019 Jul 3;31(26):265901. doi: 10.1088/1361-648X/ab13c9. Epub 2019 Mar 27.
Lysozyme is an enzyme often used as an antibacterial agent in food industries and biochemical and pharmaceutical laboratories. Immobilisation of lysozyme by encapsulating in a nanotube has received much interest as it can enhance stability of the enzyme in ambient condition. Experimentally, various types of nanotubes have been proposed as a host for lysozyme. Here, we mathematically model the immobilisation process and the interaction between lysozyme and various types of nanotubes in order to compare the effectiveness of different nanotube materials. In this paper, we consider boron nitride, carbon, silicon, silicon carbide and titania nanotubes. For each type of nanotubes, we determine the critical radius that will maximise the interaction between the lysozyme molecule and the nanotube. Our results suggest that titania nanotube stands out as the most promising candidate for lysozyme storage and delivery. The model presented here can be extended to further investigate the interaction between different types of nanotube materials and protein structures for the development of effective molecular storage.
溶菌酶是一种常用于食品工业、生物化学和制药实验室的抗菌剂。将溶菌酶包埋在纳米管中进行固定化受到了广泛关注，因为它可以提高酶在环境条件下的稳定性。在实验中，已经提出了各种类型的纳米管作为溶菌酶的宿主。在这里，我们对固定化过程和溶菌酶与各种类型的纳米管之间的相互作用进行了数学建模，以便比较不同纳米管材料的有效性。在本文中，我们考虑了氮化硼、碳、硅、碳化硅和二氧化钛纳米管。对于每种类型的纳米管，我们确定了将最大限度地提高溶菌酶分子与纳米管之间相互作用的临界半径。我们的结果表明，二氧化钛纳米管是溶菌酶储存和输送的最有前途的候选材料。这里提出的模型可以扩展到进一步研究不同类型的纳米管材料和蛋白质结构之间的相互作用，以开发有效的分子储存。
