通过固定在多壁碳纳米管上来增强溶菌酶的抗菌效果。
Increasing the antibacterial effect of lysozyme by immobilization on multi-walled carbon nanotubes.
作者信息
Merli Daniele, Ugonino Monica, Profumo Antonella, Fagnoni Maurizio, Quartarone Eliana, Mustarelli Piercarlo, Visai Livia, Grandi Marco S, Galinetto Pietro, Canton Patrizia
机构信息
Department of General Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy.
出版信息
J Nanosci Nanotechnol. 2011 Apr;11(4):3100-6. doi: 10.1166/jnn.2011.3758.
We report a facile strategy to obtain multiwalled carbon nanotubes (MWCNTs) functionalized with covalently bonded lysozyme. The functionalization procedure has been investigated by means of several techniques, including thermogravimetry, Raman spectroscopy, transmission electron microscopy, and cyclic voltammetry. A functionalization of about 1 lysozyme molecule every 4000 carbon atoms is obtained. The modified lysozyme-CNTs nanocomposite shows a significant increase of the antibacterial activity towards the Gram-positive S. aureus if compared with lysozyme in solution.
我们报道了一种简便的策略来获得共价键合溶菌酶功能化的多壁碳纳米管（MWCNTs）。通过多种技术对功能化过程进行了研究，包括热重分析、拉曼光谱、透射电子显微镜和循环伏安法。每4000个碳原子可实现约1个溶菌酶分子的功能化。与溶液中的溶菌酶相比，改性的溶菌酶 - 碳纳米管纳米复合材料对革兰氏阳性金黄色葡萄球菌的抗菌活性显著提高。
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