Department of Biotechnology and Plant Breeding, Ferdowsi University of Mashhad, Mashhad 91775-1163, Iran.
Department of Pharmacognosy; Mashhad University of Medical Sciences, Mashhad 91886-17871, Iran.
Int J Mol Sci. 2019 Mar 26;20(6):1507. doi: 10.3390/ijms20061507.
There is an urgent need to develop novel drugs for osteoporosis which occurs due to estrogen deficiency. Phytoestrogens derived from medicinal plants would be the best alternative to chemical drugs with harmful side effects. The main purpose of the present study was to investigate the effect of ferutinin compared to 17β-estradiol (E2) on bone mineralization of zebrafish larvae. Regarding the lack of publications, the histology analysis was performed after exposure to E2 to find effective treatment on bone mineralization of developing zebrafish larvae. Then, the larvae were exposed to four concentrations of ferutinin at three time points to assess the mortality, the expression of some related genes and histology of the ceratohyal and hyomandibular of treated larvae. The RT-PCR result of the treatment groups demonstrated the similar expression pattern in the larvae which were exposed to 1.25 μg/mL of ferutinin and 2 µM of E2 at 2 dpf, which confirmed the result of histology analysis. In addition, RT-qPCR of high concentration of ferutinin and E2 demonstrated that and were downregulated and upregulated when the larvae were exposed to 5 μg/mL of ferutinin and 10 µM of E2, respectively.
由于雌激素缺乏而导致的骨质疏松症，迫切需要开发新的药物。植物雌激素来源于药用植物，将成为具有有害副作用的化学药物的最佳替代品。本研究的主要目的是研究与 17β-雌二醇（E2）相比 ferutinin 对斑马鱼幼虫骨矿化的影响。由于缺乏相关出版物，在暴露于 E2 后进行组织学分析，以找到有效治疗发育中斑马鱼幼虫骨矿化的方法。然后，将幼虫暴露于四种浓度的 ferutinin 中，在三个时间点评估死亡率、某些相关基因的表达以及处理幼虫的 ceratohyal 和 hyomandibular 的组织学。处理组的 RT-PCR 结果表明，在 2 dpf 时暴露于 1.25μg/mL ferutinin 和 2μM E2 的幼虫中，表达模式相似，这证实了组织学分析的结果。此外，高浓度 ferutinin 和 E2 的 RT-qPCR 表明，当幼虫暴露于 5μg/mL ferutinin 和 10μM E2 时， 和 分别下调和上调。
