Department of Surgical, Medical, Dental and Morphological Sciences with Interest in Transplants, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.
Life Sci. 2013 May 30;92(20-21):993-1003. doi: 10.1016/j.lfs.2013.03.018. Epub 2013 Apr 10.
The phytoestrogen Ferutinin plays an important role in prevention of osteoporosis caused by ovariectomy-induced estrogen deficiency in rats, but there is no evidence of its effect on osteoblastic differentiation in vitro. In this study we investigated the effect of Ferutinin on proliferation and osteoblastic differentiation of two different human stem cells populations, one derived from the amniotic fluid (AFSCs) and the other from the dental pulp (DPSCs).
AFSCs and DPSCs were cultured in a differentiation medium for 14 or 21days with or without the addition of Ferutinin at a concentration ranging from 10(-11) to 10(-4)M. 17β-Estradiol was used as a positive drug at 10(-8)M. Cell proliferation and expression of specific osteoblast phenotype markers were analyzed.
MTT assay revealed that Ferutinin, at concentrations of 10(-8) and 10(-9)M, enhanced proliferation of both AFSCs and DPSCs after 72h of exposure. Moreover, in both stem cell populations, Ferutinin treatment induced greater expression of the osteoblast phenotype markers osteocalcin (OCN), osteopontin (OPN), collagen I, RUNX-2 and osterix (OSX), increased calcium deposition and osteocalcin secretion in the culture medium compared to controls. These effects were more pronounced after 14days of culture in both populations.
The enhancing capabilities on proliferation and osteoblastic differentiation displayed by the phytoestrogen Ferutinin make this compound an interesting candidate to promote bone formation in vivo.
植物雌激素 Ferutinin 在预防卵巢切除诱导的大鼠骨质疏松症中发挥重要作用，但尚无其在体外促进成骨细胞分化的证据。本研究旨在探讨 Ferutinin 对两种不同人干细胞群体（一种来源于羊水（AFSCs），另一种来源于牙髓（DPSCs））增殖和成骨细胞分化的影响。
将 AFSCs 和 DPSCs 在分化培养基中培养 14 或 21 天，并在 10(-11) 至 10(-4)M 浓度范围内添加或不添加 Ferutinin。17β-雌二醇以 10(-8)M 用作阳性药物。分析细胞增殖和特定成骨细胞表型标志物的表达。
MTT 测定显示，Ferutinin 在 10(-8) 和 10(-9)M 浓度下，在暴露 72 小时后可增强两种 AFSCs 和 DPSCs 的增殖。此外，在两种干细胞群体中，Ferutinin 处理诱导骨钙素（OCN）、骨桥蛋白（OPN）、I 型胶原、RUNX-2 和骨形成蛋白 2（OSX）等成骨细胞表型标志物的表达增加，与对照组相比，培养基中钙沉积和骨钙素分泌增加。在两种细胞群体中，培养 14 天后这些作用更为明显。
植物雌激素 Ferutinin 表现出的促进增殖和成骨细胞分化的能力，使其成为一种有前途的体内促进骨形成的候选化合物。
