Renji Hospital, Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China.
Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, 750004, China.
Sci Data. 2019 Mar 27;6(1):8. doi: 10.1038/s41597-019-0014-9.
Germline stem cells are germ cells at an early developmental stage, so their development is key to ensuring human reproduction. There is increasing evidence that long noncoding RNA (lncRNA) and circular RNA (circRNA) play important roles in the development of germ cells. This data descriptor provides unique lncRNA and circRNA transcriptomic information for mouse germline stem cells. Using the Illumina HiSeqx 2000 system, a total of 511,836,732 raw reads were generated. High-quality transcripts, lncRNAs, and circRNAs were identificated and quantified using the reads, and more precise annotations of lncRNAs (especially 9357 novel lncRNAs) and circRNAs were performed in the germline stem cells. We then analyzed the transcript structures, genetic variants, and the interaction between circRNA and microRNA to provide the basis for subsequent functional experiments. This comprehensive dataset will help advance data sharing and deepen our understanding of mouse germline stem cells, providing a theoretical foundation for research on germ cell development and human reproduction, among others.
生殖细胞干细胞是早期发育阶段的生殖细胞，因此它们的发育是确保人类生殖的关键。越来越多的证据表明，长非编码 RNA（lncRNA）和环状 RNA（circRNA）在生殖细胞的发育中发挥重要作用。本数据描述符提供了小鼠生殖细胞干细胞独特的 lncRNA 和 circRNA 转录组信息。使用 Illumina HiSeqx 2000 系统，共产生了 511,836,732 个原始读数。使用这些读数鉴定和定量了高质量的转录本、lncRNA 和 circRNA，并在生殖细胞干细胞中对 lncRNA（特别是 9357 个新的 lncRNA）和 circRNA 进行了更精确的注释。然后，我们分析了转录本结构、遗传变异以及 circRNA 和 microRNA 之间的相互作用，为后续的功能实验提供了基础。这个全面的数据集将有助于促进数据共享，并加深我们对小鼠生殖细胞干细胞的理解，为生殖细胞发育和人类生殖等方面的研究提供理论基础。
