Karner J, Roth E, Funovics J, Hanusch J, Walzer L R, Berger A, Meissl G
JPEN J Parenter Enteral Nutr. 1986 Jul-Aug;10(4):393-8. doi: 10.1177/0148607186010004393.
This study set out to investigate the effect of three different parenterally administered diets on the free amino acid (AA) levels in the plasma, muscle, and liver of scalded rats. Diet I consisted of AA (1.4 g/100 g weight) and a high glucose dose (6 g/100 g weight), diet II consisted of AA and a low glucose dose (1.4 g/100 g weight) and in diet III only a low glucose dose was infused. Parenteral nutrition was started on the 3rd day posttraumatically. Sampling was performed on the 7th day posttraumatically. Nitrogen balances were significantly different in all three groups, being lowest in group III. Scalded rats fed isonitrogenously, but with different amounts of glucose showed only minor changes in AA concentrations. However scalded rats fed with a nitrogen-free diet exhibited significantly reduced total muscle and liver AA levels. These decreased AA levels were due to a drop of glycine in the muscle tissue (74%) and liver (49%). Contrary to the clinical catabolic situation in scalded and starved rats, it was not intracellular glutamine but glycine which was considerably influenced by catabolism and starvation.
本研究旨在探讨三种不同的肠外营养饮食对烫伤大鼠血浆、肌肉和肝脏中游离氨基酸（AA）水平的影响。饮食I包含氨基酸（1.4 g/100 g体重）和高剂量葡萄糖（6 g/100 g体重），饮食II包含氨基酸和低剂量葡萄糖（1.4 g/100 g体重），饮食III仅输注低剂量葡萄糖。创伤后第3天开始肠外营养。创伤后第7天进行采样。三组的氮平衡存在显著差异，III组最低。等氮喂养但葡萄糖含量不同的烫伤大鼠，其氨基酸浓度仅有轻微变化。然而，无氮饮食喂养的烫伤大鼠，其肌肉和肝脏中的总氨基酸水平显著降低。这些降低的氨基酸水平是由于肌肉组织（74%）和肝脏（49%）中的甘氨酸减少所致。与烫伤和饥饿大鼠的临床分解代谢情况相反，受分解代谢和饥饿影响较大的不是细胞内谷氨酰胺，而是甘氨酸。
