Keleher Madeline Rose, Zaidi Rabab, Patel Kayna, Ahmed Amer, Bettler Carlee, Pavlatos Cassondra, Shah Shyam, Cheverud James M
1Department of Evolution, Ecology, and Population Biology, Washington University in St. Louis, St. Louis, MO USA.
2Department of Biology, Loyola University, 1032 W. Sheridan Road, Chicago, IL 60660 USA.
J Diabetes Metab Disord. 2018 Nov 22;17(2):297-307. doi: 10.1007/s40200-018-0373-3. eCollection 2018 Dec.
Obesity is linked to cognitive dysfunction in humans and rodents, and its effects can be passed on to the next generation. However, the extent of these effects is not well understood. The purpose of this study was to determine the effect of a prenatal maternal high-fat diet and an individual high-fat diet in inbred mice.
We varied maternal diet and offspring diet to test the hypothesis that a high-fat diet would increase anxiety, reduce activity levels, and impair nest-building. First, we fed a high-fat (HF) or low-fat (LF) diet to genetically identical female Small (SM/J) mice and mated them with LF males. We cross-fostered all offspring to LF-fed SM/J nurses and weaned them onto an HF or LF diet. We weighed the mice weekly and we tested anxiety with the Open Field Test, activity levels with instantaneous scan sampling, and nest building using the Deacon Scale.
Diet significantly affected weight, with HF females weighing 28.2 g (± 1.4 g SE) and LF females weighing 15.1 g (± 1.6 g SE) at 17 weeks old. The offspring's own diet had major behavioral effects. HF mice produced more fecal boli and urinations in the Open Field Test, built lower-quality nests, and had lower activity in adulthood than LF mice. The only trait that a prenatal maternal diet significantly affected was whether the offspring built their nests inside or outside of a hut.
Offspring diet, but not prenatal maternal diet, affected a wide range of behaviors in these mice.
肥胖与人类和啮齿动物的认知功能障碍有关，其影响还可遗传给下一代。然而，这些影响的程度尚不清楚。本研究的目的是确定产前母体高脂饮食和个体高脂饮食对近交系小鼠的影响。
我们改变母体饮食和后代饮食，以检验高脂饮食会增加焦虑、降低活动水平并损害筑巢能力这一假设。首先，我们给基因相同的雌性小家鼠（SM/J）喂食高脂（HF）或低脂或低脂（LF）饮食，并让它们与喂食低脂饮食的雄性小鼠交配。我们将所有后代交叉寄养给喂食低脂饮食的SM/J代乳母鼠，并在断奶后给它们喂食高脂或低脂饮食。我们每周称小鼠体重，并用旷场试验测试焦虑水平，用即时扫描取样法测试活动水平，并用迪肯量表评估筑巢能力。
饮食对体重有显著影响，17周龄时，高脂组雌性小鼠体重为28.2克（标准误±1.4克），低脂组雌性小鼠体重为15.1克（标准误±1.6克）。后代自身的饮食对行为有重大影响。在旷场试验中，高脂组小鼠产生的粪便颗粒和排尿更多，筑巢质量更低，成年后活动水平低于低脂组小鼠。产前母体饮食唯一显著影响的性状是后代在小屋内还是小屋外筑巢。
后代饮食而非产前母体饮食影响了这些小鼠的多种行为。
