Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, Nicolás Cabrera 1, 28049, Madrid, Spain.
Bioessays. 2019 Apr;41(4):e1800241. doi: 10.1002/bies.201800241.
The fate of cellular RNAs is largely dependent on their structural conformation, which determines the assembly of ribonucleoprotein (RNP) complexes. Consequently, RNA-binding proteins (RBPs) play a pivotal role in the lifespan of RNAs. The advent of highly sensitive in cellulo approaches for studying RNPs reveals the presence of unprecedented RNA-binding domains (RBDs). Likewise, the diversity of the RNA targets associated with a given RBP increases the code of RNA-protein interactions. Increasing evidence highlights the biological relevance of RNA conformation for recognition by specific RBPs and how this mutual interaction affects translation control. In particular, noncanonical RBDs present in proteins such as Gemin5, Roquin-1, Staufen, and eIF3 eventually determine translation of selective targets. Collectively, recent studies on RBPs interacting with RNA in a structure-dependent manner unveil new pathways for gene expression regulation, reinforcing the pivotal role of RNP complexes in genome decoding.
细胞内 RNA 的命运在很大程度上取决于其结构构象，这决定了核糖核蛋白（RNP）复合物的组装。因此，RNA 结合蛋白（RBP）在 RNA 的寿命中起着关键作用。用于研究 RNP 的高灵敏度细胞内方法的出现揭示了前所未有的 RNA 结合结构域（RBD）的存在。同样，与给定 RBP 相关的 RNA 靶标的多样性增加了 RNA-蛋白质相互作用的密码。越来越多的证据强调了 RNA 构象对特定 RBP 识别的生物学相关性，以及这种相互作用如何影响翻译控制。特别是，Gemin5、Roquin-1、Staufen 和 eIF3 等蛋白质中存在的非典型 RBD 最终决定了选择性靶标的翻译。总的来说，最近关于以结构依赖性方式与 RNA 相互作用的 RBP 的研究揭示了基因表达调控的新途径，加强了 RNP 复合物在基因组解码中的关键作用。
