Cape Town HVTN Immunology Laboratory, Cape Town, South Africa.
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, 98109.
Cytometry A. 2019 Jul;95(7):722-725. doi: 10.1002/cyto.a.23753. Epub 2019 Mar 28.
A 26-color staining panel was developed to profile human antigen-specific T cells in an intracellular cytokine staining (ICS) assay using peptide pools to various antigens of interest. In addition to multiple functional markers, the panel includes differentiation/activation markers and markers to assess γδ, mucosal-associated invariant T, and NK T cells as well as conventional NK cells. Panel optimization was performed using previously cryopreserved PBMC from healthy adults, and then, expression of key functional markers in the panel was cross-validated against a validated ICS assay used in the HIV Vaccine Trials Network (HVTN). The panel is currently being used to evaluate the responses to tuberculosis and malaria vaccine candidates in volunteers from different geographic areas. © 2019 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.
一个 26 色染色面板被开发出来，用于使用肽池对各种感兴趣的抗原进行细胞内细胞因子染色 (ICS) 测定，以分析人类抗原特异性 T 细胞。除了多种功能标记物外，该面板还包括分化/激活标记物和用于评估 γδ、黏膜相关不变 T 和 NK T 细胞以及常规 NK 细胞的标记物。面板优化是使用先前从健康成年人中冷冻保存的 PBMC 进行的，然后，对面板中的关键功能标记物进行交叉验证，以验证 HIV 疫苗试验网络 (HVTN) 中使用的经过验证的 ICS 测定。该面板目前正被用于评估来自不同地理区域的志愿者对结核病和疟疾候选疫苗的反应。© 2019 作者。流式细胞术部分由 Wiley 期刊出版，代表国际推进细胞术协会。
