Moncunill Gemma, Dobaño Carlota, McElrath M Juliana, De Rosa Stephen C
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, 98109; Barcelona Centre for International Health Research (CRESIB, Hospital Clínic-Universitat de Barcelona), Barcelona, Catalonia, Spain.
Cytometry A. 2015 Apr;87(4):289-92. doi: 10.1002/cyto.a.22590. Epub 2014 Nov 18.
This panel was developed to assess antigen-specific T cells using peptide pools to various antigens of interest, although other types of antigens such as recombinant proteins or whole pathogens could be considered using different stimulation times. In addition to multiple functional markers, the panel includes differentiation markers and markers to assess follicular helper T cells and NK cells (Table 1). It was optimized using cryopreserved peripheral blood mononuclear cells (PBMC) from human immunodeficiency virus (HIV) uninfected and HIV infected adults with known cytomegalovirus (CMV) responses and it underwent assay qualification. The panel is being used to evaluate the responses to HIV and malaria vaccine candidates in adults and children from different geographic areas.
该检测板旨在通过使用针对各种感兴趣抗原的肽库来评估抗原特异性T细胞，不过对于其他类型的抗原，如重组蛋白或完整病原体，可考虑采用不同的刺激时间。除了多种功能标志物外，该检测板还包括分化标志物以及用于评估滤泡辅助性T细胞和自然杀伤细胞的标志物（表1）。它使用来自未感染人类免疫缺陷病毒（HIV）和已知巨细胞病毒（CMV）反应的HIV感染成年人的冷冻保存外周血单核细胞（PBMC）进行了优化，并经过了检测鉴定。该检测板正用于评估来自不同地理区域的成人和儿童对HIV和疟疾候选疫苗的反应。
